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Glucocorticoid Isomers on Raptor Biphenyl by LC-MS/MS

LC_CF0659
PeakstR (min)Precursor IonProduct IonProduct Ion
1.Prednisolone2.53361.2343.2325.1
2.Prednisone3.07359.1341.1147.1
3.Betamethasone4.64393.1373.191.2
4.Dexamethasone4.87393.1373.1355.2
ColumnRaptor Biphenyl (cat.# 9309A12)
Dimensions:100 mm x 2.1 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Temp.:40 °C
Sample
Diluent:Water:methanol (80:20)
Conc.:20 ng/mL
Inj. Vol.:5 µL
Mobile Phase
A:Water + 0.1% formic acid
B:Acetonitrile + 0.1% formic acid
Time (min)Flow (mL/min)%A%B
0.000.67525
6.000.67525
DetectorMS/MS
Ion Mode:ESI+
Mode:MRM
InstrumentUHPLC