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Morphine and Metabolites on Force Biphenyl by UHPLC-MS/MS

LC_CF0677
PeakstR (min)Precursor IonProduct IonProduct Ion
1.Morphine-3-β-D-glucuronide0.70462.1286.0152.1
2.Morphine1.26286.1164.9152.0
3.Morphine-6-β-D-glucuronide1.34462.1286.0152.1
4.Morphine-N-oxide1.41302.1284.9161.9
5.6-Acetylmorphine1.86328.1211.1165.0
ColumnForce Biphenyl (cat.# 9629252)
Dimensions:50 mm x 2.1 mm ID
Particle Size:1.8 µm
Temp.:35 °C
Standard/Sample
Diluent:0.1% Formic acid in water
Conc.:50 ng/mL
Inj. Vol.:5 µL
Mobile Phase
A:0.1% Formic acid in water
B:0.1% Formic acid in methanol
Time (min)Flow (mL/min)%A%B
0.000.58515
0.500.58515
2.000.53070
2.010.58515
4.000.58515
DetectorMS/MS
Ion Mode:ESI+
Mode:MRM
InstrumentUHPLC