The Analysis of Morphine & Metabolites in Urine without Hydrolysis on Force Biphenyl

	Peaks	t_R (min)	Precursor Ion	Product Ion	Product Ion
1.	Morphine-3-β-D-glucuronide	0.61	462.1	286.0	152.1
2.	Morphine	1.06	286.1	152.0	164.9
3.	Morphine-6-β-D-glucuronide	1.17	462.1	286.0	152.1
4.	Morphine-N-oxide	1.30	302.1	284.9	161.9
5.	6-Monoacetylmorphine	1.81	328.1	211.1	165.0
6.	Hydrocodone	1.91	300.3	199.2	128.3

Column

Force Biphenyl (cat.# 9629252)

Dimensions:

50 mm x 2.1 mm ID

Particle Size:

1.8 µm

Guard Column:

UltraShield UHPLC precolumn filter, 0.2 µm frit (cat.# 25809)

Temp.:

40 °C

Standard/Sample

Conc.:

A 750 ng/mL standard was prepared in 1 mL of urine. 100 μL of the fortified urine standard was diluted with 900 μL of 0.1% formic acid in water prior to analysis.

Inj. Vol.:

2 µL

Mobile Phase

0.1% Formic acid in water

0.1% Formic acid in methanol

Time (min)	Flow (mL/min)	%A	%B
0.00	0.5	85	15
0.50	0.5	85	15
2.00	0.5	30	70
2.01	0.5	85	15
4.00	0.5	85	15

Detector	MS/MS
Ion Mode:	ESI+
Mode:	MRM
Instrument	UHPLC

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