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Steroid Panel Analysis on Force Biphenyl

LC_CF0702
PeakstR (min)Q1Q3 QuantifierQ3 Qualifier
1.Cortisol2.3363.3121.2327.2
2.Estradiol2.6255.3159.1133.1
3.11-Deoxycortisol4.0347.4109.297.1
4.DHEA4.3271.3253.2213.2
5.Boldenone4.4287.2121.2135.2
6.Corticosterone4.4347.4329.2121.1
7.Stanozolol4.4329.381.195.1
8.Epitestosterone4.5289.2109.297.1
PeakstR (min)Q1Q3 QuantifierQ3 Qualifier
9.17-OH-progesterone4.8331.397.2109.5
10.Testosterone5.0289.2109.197.0
11.Trenbolone5.1303.3109.297.1
12.Methyltestosterone5.1271.2253.1199.2
13.Nandrolone5.3275.2109.2257.3
14.25-Hydroxyvitamin D36.1383.3365.3107.1
15.Androstenedione6.2287.297.1109.2
16.Progesterone7.5315.3109.297.1
ColumnForce Biphenyl LC Column (cat.# 9629512)
Dimensions:100 mm x 2.1 mm ID
Particle Size:5 µm
Pore Size:100 Å
Temp.:30 °C
SampleSteroids panel
Diluent:50:50 Methanol:water
Conc.:5 ng/mL
Inj. Vol.:50 µL
Mobile Phase
A:Water, 0.1% formic acid
B:Methanol, 0.1% formic acid
Time (min)Flow (mL/min)%A%B
0.000.43070
8.000.40100
8.010.43070
10.000.43070
Max Pressure:250 bar
DetectorMS/MS
Ion Mode:ESI+
Mode:Scheduled MRM
InstrumentUHPLC
Notes