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Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine on Raptor EtG/EtS

LC_CF0704
PeakstR (min)Precursor IonProduct IonProduct Ion
1.Ethyl-β-D-glucuronide-d50.86225.984.9-
2.Ethyl-β-D-glucuronide0.87220.884.974.8
3.Ethyl sulfate-d52.14129.797.7-
4.Ethyl sulfate2.17124.796.879.7
ColumnRaptor EtG/EtS (cat.# 9325A12)
Dimensions:100 mm x 2.1 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Guard Column:UltraShield UHPLC precolumn filter, 0.2 µm frit (cat.# 25809)
Temp.:35 °C
Sample
Diluent:0.1% Formic acid in water
Conc.: A 500 ng/mL standard was prepared in urine. 50 µL of the standard was diluted with 950 µL of a working internal standard (25 ng/mL EtS-d5/100 ng/mL EtG-d5 in 0.1% formic acid in water). The sample was vortexed at 3,500 rpm for 10 seconds to mix. The sample was then centrifuged at 3,000 rpm for 5 minutes at 10 °C. The autosampler needle was adjusted to inject from the supernatant.
Inj. Vol.:10 µL
Mobile Phase
A:0.1% Formic acid in water
B:0.1% Formic acid in acetonitrile
Time (min)Flow (mL/min)%A%B
0.000.5955
2.500.56535
2.510.5955
4.000.5955
DetectorMS/MS
Ion Mode:ESI-
Mode:MRM
InstrumentHPLC
NotesReference Standards
Ethyl-β-D-glucuronide (cat.# 34101)
Ethyl-β-D-glucuronide-d5 (cat.# 34102)
Ethyl sulfate sodium salt (cat.# 34103)
Ethyl sulfate-d5 sodium salt (cat.# 34104)