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Bile Acids with Isomer Separation in Human Plasma on Raptor C18 (1.8 μm) by LC-MS/MS

LC_CF0712
ColumnRaptor C18 (cat.# 9304252)
Dimensions:50 mm x 2.1 mm ID
Particle Size:1.8 µm
Pore Size:90 Å
Guard Column:UltraShield UHPLC precolumn filter 0.2 µm (cat.# 25810)
Temp.:60 °C
Sample
Diluent:70:30 Water:methanol
Inj. Vol.:3 µL
Mobile Phase
A:5 mM Ammonium acetate in water
B:50:50 Acetonitrile:methanol
Time (min)Flow (mL/min)%A%B
0.000.56535
2.000.56040
2.500.55545
3.500.55050
4.600.54555
5.700.52080
5.900.8*595
6.500.8*595
6.510.56535
8.500.56535
DetectorMS/MS
Ion Mode:ESI-
Mode:MRM
InstrumentUHPLC
Notes*The flow rate was increased to 0.8 mL/min to more thoroughly flush phospholipids from the analytical column, thereby reducing matrix effects.