Welcome to the New Restek.com! Take a tour of the new site and feel free to send us your feedback.
Your web browser will no longer be supported by Restek.com as of 30 June 2021.
To avoid any interruption in access or functionality, install a current-generation web browser now. Learn more.

Creatine and Creatinine in Human Urine on Raptor HILIC-Si by LC-MS/MS

LC_CF0723
PeakstR (min)Precursor IonProduct IonProduct Ion
1.Creatinine0.537114.044.386.0
2.Creatine1.240132.143.390.2
ColumnRaptor HILIC-Si (cat.# 9310A52)
Dimensions:50 mm x 2.1 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Guard Column:UltraShield UHPLC precolumn filter 0.2 µm (cat.# 25810)
Temp.:40 °C
Sample
Diluent:20:80 Water:acetonitrile
Conc.: Endogenous levels
Inj. Vol.:0.2 µL
Mobile Phase
A:5 mM Ammonium formate in 20:80 water:acetonitrile
Time (min)Flow (mL/min)%A
0.000.5100
1.5Stop
DetectorMS/MS
Ion Mode:ESI+
Mode:MRM
InstrumentUHPLC
NotesSample Preparation:
Endogenous levels of creatinine and creatine in human urine were determined using a simple dilute-and-shoot method. A 50 μL aliquot of human urine was mixed with 950 μL acetonitrile. After vortexing and centrifuging at 4,300 rpm for 10 min, 10 μL of the supernatant was added to the 1,490 μL of 80% acetonitrile in water. Centrifugation was performed again before injection.