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Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Oral Fluid on Raptor EtG/EtS

LC_CF0725
PeakstR (min)Conc.
(ng/mL)
Precursor IonProduct IonProduct Ion
1.Ethyl-β-D-glucuronide-d50.77100225.984.7-
2.Ethyl-β-D-glucuronide0.78500220.974.985.0
3.Ethyl sulfate-d51.75100129.897.7-
4.Ethyl sulfate1.78500124.896.879.7
ColumnRaptor EtG/EtS (cat.# 9325A12)
Dimensions:100 mm x 2.1 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Guard Column:UltraShield UHPLC precolumn filter (cat.# 25809)
Temp.:30 °C
SampleEthyl-β-D-glucuronide (EtG) (cat.# 34101)
Ethyl-β-D-glucuronide-d5 (EtG-d5) (cat.# 34102)
Ethyl sulfate sodium salt (EtS) (cat.# 34103)
Ethyl sulfate-d5 sodium salt (EtS-d5) (cat.# 34104)
Diluent:0.1% Formic acid in water
Conc.:500 ng/mL
Inj. Vol.:10 µL
Mobile Phase
A:0.1% Formic acid in water
B:0.1% Formic acid in acetonitrile
Time (min)Flow (mL/min)%A%B
0.000.5955
2.500.56535
2.510.5955
4.000.5955
DetectorMS/MS
Ion Mode:ESI-
Mode:MRM
InstrumentHPLC
NotesA 500 ng/mL standard was prepared in oral fluid. 50 µL of the standard was diluted with 950 µL of a working internal standard (100 ng/mL EtG-d5 and EtS-d5 in 0.1% formic acid in water). The sample was vortexed at 3000 rpm for 10 seconds to mix. The sample was then centrifuged at 3000 rpm for 10 minutes at 10 °C. The autosampler needle was adjusted to inject from the supernatant.