Welcome to the New Restek.com! Take a tour of the new site and feel free to send us your feedback.
Your web browser will no longer be supported by Restek.com as of 30 June 2021.
To avoid any interruption in access or functionality, install a current-generation web browser now. Learn more.

EtG/EtS in UTAK EtG Plus Level 1 Urine Control on Raptor EtG/EtS by LC-MS/MS

LC_CF0726
PeakstR (min)Conc.
(ng/mL)
Precursor IonProduct IonProduct Ion
1.Ethyl-β-D-glucuronide0.81500221.2075.185.1
2.Ethyl-β-D-glucuronide-d50.81100226.2085.00-
3.Ethyl sulfate-d51.84100130.1098.05-
4.Ethyl sulfate1.87200125.1097.1080.00
ColumnRaptor EtG/EtS (cat.# 9325A12)
Dimensions:100 mm x 2.1 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Guard Column:UltraShield UHPLC precolumn filter, 0.2 μm frit (cat.# 25809)
Temp.:35 °C
Sample
Ethyl-β-D-glucuronide-d5 (EtG-d5) (cat.# 34102)
Ethyl sulfate-d5 sodium salt (EtS-d5) (cat.# 34104)
Diluent:0.1% Formic acid in water
Conc.: UTAK EtG Plus urine quality control (200 ng/mL EtS and 500 ng/mL EtG)
Inj. Vol.:10 µL
Mobile Phase
A:0.1% Formic acid in water
B:0.1% Formic acid in acetonitrile
Time (min)Flow (mL/min)%A%B
0.000.5955
2.500.56535
2.510.5955
4.000.5955
DetectorMS/MS
Ion Source:Electrospray
Ion Mode:ESI-
Mode:MRM
InstrumentHPLC
NotesUTAK EtG Plus Level 1 control contains EtG at 500 ng/mL and EtS at 200 ng/mL concentrations. A 50 μL aliqout was taken from the the Level 1 control and diluted 20 times with 950 μL of a working internal standard (100 ng/mL EtG-d5 and EtS-d5 in 0.1% formic acid in water). The sample was vortexed at 3000 rpm for 10 seconds to mix. The sample was then centrifuged at 3000 rpm for 10 minutes at 10 °C and 10 μL was injected for LC-MS/MS analysis.