Welcome to the New Restek.com! Take a tour of the new site and feel free to send us your feedback.
Your web browser will no longer be supported by Restek.com as of 30 June 2021.
To avoid any interruption in access or functionality, install a current-generation web browser now. Learn more.

22 Acylcarnitines and 13 Amino acids (Endogenous) in Dried Blood Spots with 16 Deuterated Internal Standards on Raptor HILIC-Si EXP Guard Cartridge Column by LC-MS/MS

LC_CF0759
PeakstR (min)Precursor IonProduct Ion
1.Phenylalanine0.20166.0120.1
2.Leucine0.21132.186.0
3.Leucine-d30.21135.289.1
4.Isoleucine0.21132.186.1
5.Tyrosine0.22182.191.0
6.Methionine0.24150.156.1
7.Methionine-d30.24153.2107.1
8.Valine0.27118.172.0
9.C20-Eicosanoyl-L-carnitine0.27456.485.1
10.C18-Stearoyl-L-carnitine0.28428.385.1
11.C18-Stearoyl-L-carnitine-d30.29431.485.1
12.C18:1 Oleoyl-L-carnitine0.29426.485.1
13.C18:2 Linoleoyl-L-carnitine0.30424.385.1
14.C16-Palmitoyl-L-carnitine0.30400.385.1
15.C16-Palmitoyl-L-carnitine-d30.31403.385.1
16.C16:1 Palmitolelyl-L-carnitine0.31398.385.1
17.C14-Myristoyl-L-carnitine0.32372.385.1
18.C14-Myristoyl-L-carnitine-d30.33375.385.1
19.C14:1 Tetradecenoyl-L-carnitine0.33370.385.1
20.C14:2-Tetradecadienoyl-L-carnitine0.33368.385.1
21.Proline0.33116.070.1
22.C12-Lauroyl-L-carnitine0.35344.385.1
23.C12-Lauroyl-L-carnitine-d30.35347.385.1
24.Alanine0.3690.144.1
25.Alanine-d40.3594.148.1
PeakstR (min)Precursor IonProduct Ion
26.C10-Decanoyl-L-carnitine0.38316.385.1
27.C10-Decanoyl-L-carnitine-d30.39319.285.1
28.C8-Octanoyl-L-carnitine0.43288.385.1
29.C8-Octanoyl-L-carnitine-d30.43291.285.1
30.C7-Heptanoyl-L-carnitine0.45274.285.1
31.C6-Hexanoyl-L-carnitine-d30.46263.285.1
32.C6-Hexanoyl-L-carnitine0.47260.285.1
33.Glutamine0.45147.184.1
34.C5-Valeryl-L-carnitine0.48246.285.1
35.C5-Valeryl-L-carnitine-d30.49249.185.1
36.C5-Isovaleryl-L-carnitine0.49246.185.1
37.C5-Isovaleryl-L-carnitine-d30.49249.285.1
38.2-Methylbutyryl-L-carnitine0.49246.285.1
39.C5:1-Tiglyl-L-carnitine0.50244.285.1
40.C4-Butyryl-L-carnitine0.51232.285.1
41.C4-Butyryl-L-carnitine-d30.51235.285.1
42.C4-Isobutyryl-L-carnitine0.51232.185.1
43.C4-Isobutyryl-L-carnitine-d30.51235.185.1
44.Citrulline0.51176.1113.1
45.Glutamic acid0.55148.183.9
46.C3-Propionyl-L-carnitine0.54218.185.1
47.C3-Propionyl-L-carnitine-d30.54221.285.1
48.C2-Acetyl-L-carnitine0.56204.185.1
49.C2-Acetyl-L-carnitine-d30.56207.185.1
50.Arginine0.66175.270.1
51.Ornithine0.69133.170.1
All analytes were present endogenously at varying concentrations in whole blood, except the deuterated internal standards.
ColumnRaptor HILIC-Si EXP guard cartridge column (cat.# 9310A0252)
Dimensions:5 mm x 2.1 mm ID
Particle Size:2.7 µm
Temp.:45 °C
Sample
Diluent:85:15 Acetonitrile:water (v/v)
Conc.: Endogenous levels
Inj. Vol.:2.0 µL
Mobile Phase
A:30 mM Ammonium formate in water
B:Acetonitrile
Time (min)Flow (mL/min)%A%B
0.000.51585
0.40.57030
0.410.51585
1.20.51585
DetectorMS/MS
Ion Source:Electrospray
Ion Mode:ESI+
InstrumentUHPLC
Notes50 µL of whole blood was spotted on to Whatman 903 neonatal protein saver cards, which were then dried for 1 hour at room temperature. A 3.0 mm disk (∼3.0 µL whole blood) was punched out of the dried spot and into a 2.0 mL Eppendorf tube. 200 µL of 85:15 acetonitrile:water (v/v) that was fortified with known concentrations of stable isotope-labeled internal standards was added, and then the sample was vortexed and incubated for 20 minutes at room temperature on a microplate shaker at a speed of 400 rpm. The sample was then centrifuged for 10 minutes at 4000 rpm, and 150 µL of the supernatant was filtered using a Thomson SINGLE StEP Nano filter vial (cat.# 25882) prior to LC-MS/MS analysis.