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Psilocybin and Psilocin in Methanol Sample Diluent on Raptor HILIC-Si by LC-MS/MS

LC_CF0765
PeakstR (min)Conc.
(ng/mL)
Precursor IonProduct Ion 1Product Ion 2
1.Psilocin1.13100205.1160.1115.0
2.Psilocybin1.75100285.1240.0205.2
ColumnRaptor HILIC-Si (cat.# 9310A52)
Dimensions:50 mm x 2.1 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Temp.:40 °C
Sample
Diluent:10 mM Ammonium formate in 100% methanol
Conc.:100 ng/mL
Inj. Vol.:2 µL
Mobile Phase
A:Water, 10 mM ammonium formate
B:90:10 Acetonitrile:water (v/v), 10 mM ammonium formate
Time (min)Flow (mL/min)%A%B
0.000.50100
0.200.50100
1.700.5955
1.710.50100
3.000.50100
DetectorMS/MS
Ion Source:Electrospray
Ion Mode:ESI+
Mode:MRM
NotesA 100 ng/mL standard mix of psilocin and psilocybin was prepared in 10 mM ammonium formate in 100% methanol. The solution was vortexed at 3000 rpm for 10 seconds to mix, and then the supernatant was injected for LC-MS/MS analysis.