Barbiturates, THC-COOH, and THCA-A on Force Biphenyl by LC-MS/MS

	Peaks	t_R (min)	Conc. (ng/mL)	Precursor	Product 1	Product 2
1.	Phenobarbital	2.01	500	230.9	42.1	-
2.	Butalbital	2.14	500	223.0	180.2	42.1
3.	Amobarbital	2.56	500	225.0	182.2	42.1
4.	Pentobarbital	2.62	500	225.0	182.2	42.1
5.	Secobarbital	2.81	500	337.0	194.2	42.1
6.	THC-COOH	3.26	5	343.0	299.2	245.1
7.	THCA-A	3.58	5	357.1	313.3	245.1

Column

Force Biphenyl (cat.# 962935E)

Dimensions:

50 mm x 3.0 mm ID

Particle Size:

3 µm

Pore Size:

100 Å

Guard Column:

Force Biphenyl EXP guard column cartridge 5 mm, 3 mm ID, 5 µm (cat.# 962950253)

Temp.:

30 °C

Standard/Sample

Phenobarbital (cat.# 34037)

Pentobarbital (cat.# 34036)

Butalbital (cat.# 34032)

Secobarbital (cat.# 34038)

(±)11-Nor-9-carboxy-D9-THC (cat.# 34068)

d9-Tetrahydrocannabinolic acid A (cat.# 34111)

Diluent:

1:9 Urine:water

Inj. Vol.:

1 µL

Mobile Phase

Water, 0.1% formic acid

Methanol, 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.8	55	45
2.00	0.8	40	60
2.50	0.8	0	100
4.00	0.8	0	100
4.01	0.8	55	45
5.00	0.8	55	45

Detector	MS/MS
Ion Source:	Electrospray
Ion Mode:	ESI-
Sample Preparation	A 100 μL aliquot of urine was spiked with analytes and diluted with 900 μL of MS water in a 2 mL screw-thread vial (cat.# 21143) and capped with a short-cap, screw-vial closure (cat.#24498). The sample was vortexed for 10 seconds and injected for LC-MS/MS analysis.
Notes	The analytical and guard columns were equipped with an UltraShield precolumn filter (cat. 25809).

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