EPA 8310 PAH Mix on Pinnacle II PAH (FLD)

Column

Pinnacle II PAH, custom (cat.# 921946E)

Dimensions:

150 mm x 3.0 mm ID

Particle Size:

4 µm

Pore Size:

110 Å

Temp.:

30 °C

Standard/Sample

EPA Method 8310 PAH mixture (cat.# 31841)

Diluent:

Acetonitrile

Conc.:

30 ng/mL (ppb)

Inj. Vol.:

5 µL

Mobile Phase

Water

Acetonitrile

Time (min)	Flow (mL/min)	%A	%B
0.00	1.1	55	45
6.50	1.1	40	60
8.00	1.1	30	70
12.00	1.1	0	100
15.00	1.1	0	100
15.01	1.1	55	45
17.00	1.1	55	45

Detector

Shimadzu RF-10AXL

Time (min)	Wavelength Ex (nm)	Wavelength Em (nm)	Gain
0	270	330	16x
7.50	250	370	16x
9.20	330	430	4x
11.00	270	390	1x
12.20	290	430	1x
14.70	370	460	4x

Instrument

Shimadzu Prominence

Notes

RF-10AXL sensitivity: medium
Acenaphthylene is included in PAH standard mix (cat.# 31841) but does not fluoresce.

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