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Phenoxyacid Herbicides on Ultra Cyano (Overlay)

LC_EV0528
PeakstR (min)Conc.
(µg/mL)
1.Dalapon3.49100
2.Picloram5.98100
3.Dicamba8.68100
4.Chloramben9.04100
5.4-Nitrophenol9.71100
6.2,4-Dichlorophenylacetic acid12.5340
7.Bentazon12.70100
8.(4-Chloro-o-toloxy)acetic acid13.07100
9.3,5-Dichlorobenzoic acid13.41100
10.2,4-D13.49100
11.Mecoprop14.68100
PeakstR (min)Conc.
(µg/mL)
12.Dichlorprop14.88100
13.2,4,5-T15.66100
14.2,4-DB15.83100
15.2,4,5-TP16.73100
16.Phenol, pentachloro-19.42100
17.Acifluorfen19.47100
18.Dinoseb20.00100
19.2,4-D Butoxyethyl ester20.78200
20.2,4,5-T Butoxyethyl ester21.97200
21.2,4,5-T Butyl ester22.29200
22.2,4-D 2-Ethylhexyl ester24.12200
ColumnUltra Cyano (cat.# 9106565)
Dimensions:150 mm x 4.6 mm ID
Particle Size:5 µm
Pore Size:100 Å
Temp.:30 °C
SampleChlorinated acids by HPLC, mix B (cat.# 32430)
Chlorinated acids by HPLC, mix A (cat.# 32431)
Dalapon (cat.# 32432)
2,4-Dichlorophenylacetic acid (cat.# 32439)
Diluent:Water
Inj. Vol.:5 µL
Mobile Phase
A:0.05% H3PO4 in water
B:Acetonitrile
Time (min)Flow (mL/min)%A%B
0.0019010
2513565
DetectorUV/Vis @ 220, 0 nm
InstrumentShimadzu UFLCXR
NotesOverlay of 5 injections
1. Black trace = cat.# 32430
2. Red trace = cat.# 32431
3. Purple trace = cat.# 32432
4. Green trace = cat.# 32439
5. Blue trace = metabolite mix