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Perfluorinated Alkyl Acids on Raptor C18 by LC-MS/MS for EPA Method 537

LC_EV0548
PeakstR (min)Conc.
(ng/mL)
Precursor IonProduct Ion
1.Perfluorobutanesulfonic acid (PFBS)1.9810299.1079.99
2.Perfluoro-n-[1,2-13C2]hexanoic acid (13C-PFHxA)2.9210315.13270.13
3.Perfluorohexanoic acid (PFHxA)2.9310313.10269.12
4.Perfluoroheptanoic acid (PFHpA)4.005363.16319.09
5.Perfluorohexanesulfonic acid (PFHxS)4.1410399.1379.98
6.Perfluoro-[1,2-13C2]octanoic acid (13C-PFOA)4.835415.13370.10
7.Perfluorooctanoic acid (PFOA)4.835413.16369.10
8.Perfluorononanoic acid (PFNA)5.505463.16419.19
9.Perfluoro-1-[1,2,3,4-13C4]octanesulfonic acid (13C-PFOS)5.545503.1379.98
10.Perfluorooctanesulfonic acid (PFOS)5.5410499.1779.98
PeakstR (min)Conc.
(ng/mL)
Precursor IonProduct Ion
11.Perfluoro-n-[1,2-13C2]decanoic acid (13C-PFDA)6.065515.17470.17
12.Perfluorodecanoic acid (PFDA)6.075513.17469.16
13.N-deuteriomethylperfluoro-1-octanesulfonamidoacetic acid (d3-NMeFOSAA)6.285573.23419.15
14.N-methyl perfluorooctanesulfonamidoacetic acid (NMeFOSAA)6.285570.20419.17
15.N-deuterioethylperfluoro-1-octanesulfonamidoacetic acid (d5-NEtFOSAA)6.525589.27419.11
16.N-ethyl perfluorooctanesulfonamidoacetic acid (NEtFOSAA)6.525584.20419.18
17.Perfluoroundecanoic acid (PFUnA)6.565563.23519.24
18.Perfluorododecanoic acid (PFDoA)6.985613.23569.19
19.Perfluorotridecanoic acid (PFTrDA)7.355663.23619.21
20.Perfluorotetradecanoic acid (PFTA)7.675713.23669.23
ColumnRaptor C18 (cat.# 9304552)
Dimensions:50 mm x 2.1 mm ID
Particle Size:5 µm
Pore Size:90 Å
Temp.:40 °C
Sample
Diluent:96:4 Methanol:water
Conc.:5–10 ng/mL
Inj. Vol.:2 µL
Mobile Phase
A:5 mM ammonium acetate in water
B:Methanol
Time (min)Flow (mL/min)%A%B
0.000.47525
8.000.41585
8.010.47525
9.000.47525
DetectorMS/MS
Ion Source:Electrospray
Ion Mode:ESI-
Mode:MRM
InstrumentWaters ACQUITY