Perfluorinated Alkyl Acids (PFBS, PFOA, PFOS, and HFPO-DA/GenX) on Raptor C18 5 μm

	Peaks	t_R (min)	Conc. (ng/mL)	Precursor Ion	Product Ion
1.	Perfluorobutanesulfonic acid (PFBS)	1.56	10	298.8	79.9
2.	Hexafluoropropylene oxide-dimer acid (HFPO-DA/GenX)	2.02	10	328.9	284.9
3.	Perfluorooctanoic acid (PFOA)	2.47	5	413.1	368.9
4.	Perfluorooctanesulfonic acid (PFOS)	2.65	10	498.8	80.0

Column

Raptor C18 (cat.# 9304552)

Dimensions:

50 mm x 2.1 mm ID

Particle Size:

5 µm

Pore Size:

90 Å

Temp.:

40 °C

Standard/Sample

Diluent:

75:25 Water:methanol

Conc.:

5-10 ng/mL

Inj. Vol.:

5 µL

Mobile Phase

Water, 5 mM ammonium acetate

Methanol

Time (min)	Flow (mL/min)	%A	%B
0.00	0.4	75	25
3.00	0.4	5	95
3.01	0.4	75	25
4.00	0.4	75	25

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