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Ultra-short Chain, Legacy, and Alternative PFAS on Raptor C18 in Reagent Water

LC_EV0555
PeakstR (min)Conc.
(ng/L)
Precursor IonProduct Ion
1.Perfluoropropanoic acid (PFPrA)2.7480162.9119.0
2.Perfluorobutanoic acid (PFBA)4.6980212.8169.0
3.Perfluoropropanesulfonic acid (PFPrS)5.1380248.879.6
4.Perfluorobutanesulfonic acid (PFBS)6.1480298.879.9
5.Perfluoro-n-[1,2-13C2]hexanoic acid (13C2-PFHxA)6.7550314.9270.0
6.Hexafluoropropylene oxide-dimer acid (HFPO-DA)6.9280285.0168.9
PeakstR (min)Conc.
(ng/L)
Precursor IonProduct Ion
7.Ammonium 4,8-dioxa-3H-perfluorononanoate (ADONA)7.3380376.9250.7
8.Perfluorooctanoic acid (PFOA)7.7080413.1368.9
9.Perfluoro-[1,2-13C2]octanoic acid (13C2-PFOA)7.7050415.0370.0
10.Perfluorooctanesulfonic acid (PFOS)8.0180498.880.0
11.Perfluoro-[1,2,3,4-13C4]octanesulfonic acid (13C4-PFOS)8.0150503.080.0
12.9-Chlorohexadecafluoro-3-oxanonane-1-sulfonate (9Cl-PF3ONS)8.1580530.8350.7
13.11-Chloroeicosafluoro-3-oxanonane-1-sulfonate (11Cl-PF3OUdS)8.6180630.7451.0
ColumnRaptor C18 (cat.# 9304A1E)
Dimensions:100 mm x 3 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Temp.:40 °C
Sample
Conc.:80 ppt
Inj. Vol.:10 µL
Mobile Phase
A:Water, 5 mM ammonium acetate
B:Methanol
Time (min)Flow (mL/min)%A%B
0.000.258020
7.000.25595
9.000.25595
9.010.258020
11.00.258020
DetectorMS/MS
Ion Mode:ESI-
Mode:MRM
InstrumentUHPLC
NotesA PFAS delay column (cat.# 27854) was installed between the pump mixer and the injector.

In a polypropylene vial, 250 µL of reagent water (fortified at 80 ppt) was mixed with 250 µL of 40:60 reagent water:methanol and 5 µL of internal standard solution (5 ng/mL of 13C2-PFHxA, 13C2-PFOA, 13C4-PFOS in methanol). The vial was capped with a polyethylene cap prior to analysis.