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EPA Method 537.1 on Raptor C18

LC_EV0560
PeakstR (min)Conc.
(ng/mL)
Precursor IonProduct Ion
1.Perfluorobutanesulfonic acid (PFBS)2.0610298.879.9
2.Perfluoro-n-[1,2-13C2]hexanoic acid (13C2-PFHxA)3.035314.9270.0
3.Perfluorohexanoic acid (PFHxA)3.045312.9268.7
4.Tetrafluoro-2-heptafluoropropoxy-13C3-propanoic acid (13C3-HFPO-DA)3.365286.8168.7
5.Hexafluoropropylene oxide dimer acid (HFPO-DA)3.385285.0168.9
6.Perfluoroheptanoic acid (PFHpA)4.095362.8318.8
7.Perfluorohexanesulfonic acid (PFHxS)4.2210398.879.9
8.4,8-Dioxa-3H-perfluorononanoic acid (ADONA)4.245376.9250.7
9.Perfluoro-[1,2-13C2]octanoic acid (13C2-PFOA)4.885415.0370.0
10.Perfluorooctanoic acid (PFOA)4.905413.1368.9
11.Perfluorononanoic acid (PFNA)5.545462.9418.9
12.Perfluoro-1-[1,2,3,4-13C4]octanesulfonic acid (13C4-PFOS)5.5710503.080.0
PeakstR (min)Conc.
(ng/mL)
Precursor IonProduct Ion
13.Perfluorooctanesulfonic acid (PFOS)5.5810498.980.0
14.9-Chlorohexadecafluoro-3-oxanone-1-sulfonic acid (9Cl-PF3ONS)5.885530.8350.7
15.Perfluoro-n-[1,2-13C2]decanoic acid (13C2-PFDA)6.085514.9469.9
16.Perfluorodecanoic acid (PFDA)6.085512.9469.0
17.N-deuteriomethylperfluoro-1-octanesulfonamidoacetic acid (d3-NMeFOSAA)6.2810572.9418.8
18.N-methyl perfluorooctanesulfonamidoacetic acid (NMeFOSAA)6.3010569.8418.8
19.N-deuterioethylperfluoro-1-octanesulfonamidoacetic acid (d5-NEtFOSAA)6.5110588.9418.8
20.N-ethyl perfluorooctanesulfonamidoacetic acid (NEtFOSAA)6.5210583.8418.8
21.Perfluoroundecanoic acid (PFUnA)6.555562.9518.8
22.11-chloroeicosafluoro-3-oxaundecane-1-sulfonic acid (11Cl-PF3OUdS)6.775630.7451.0
23.Perfluorododecanoic acid (PFDoA)6.955612.7568.9
24.Perfluorotridecanoic acid (PFTrDA)7.305662.7618.8
25.Perfluorotetradecanoic acid (PFTA)7.605712.7668.7
ColumnRaptor C18 (cat.# 9304A52)
Dimensions:50 mm x 2.1 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Temp.:40 °C
Sample
Diluent:96:4 Methanol:water
Conc.:5-10 ng/mL
Inj. Vol.:2 µL
Mobile Phase
A:Water, 5 mM ammonium acetate
B:Methanol
Time (min)Flow (mL/min)%A%B
0.000.47030
8.000.41090
8.010.47030
10.00.47030
DetectorMS/MS
Ion Mode:ESI-
Mode:MRM
InstrumentHPLC