Welcome to the New Restek.com! Take a tour of the new site and feel free to send us your feedback.
Your web browser will no longer be supported by Restek.com as of 30 June 2021.
To avoid any interruption in access or functionality, install a current-generation web browser now. Learn more.

Laboratory Fortified Blank (LFB) at Midrange (40 ppt) for EPA 537.1 on Raptor C18

LC_EV0573
PeakstR (min)Conc.
(ng/L)
Precursor IonProduct Ion
1.Perfluorobutanesulfonic acid (PFBS)2.0840299.080.0
2.Perfluoro-n-[1,2-13C2]hexanoic acid (13C2-PFHxA)3.1120315.1270.1
3.Perfluorohexanoic acid (PFHxA)3.1140313.2269.0
4.Tetrafluoro-2-heptafluoropropoxy-13C3-propanoic acid (13C3-HFPO-DA)3.4720332.1287.3
5.Hexafluoropropylene oxide dimer acid (HFPO-DA)3.4740328.9284.9
6.Perfluoroheptanoic acid (PFHpA)4.1940363.2319.2
7.Perfluorohexanesulfonic acid (PFHxS)4.3340399.279.9
8.4,8-Dioxa-3H-perfluorononanoic acid (ADONA)4.3440376.9251.0
9.Perfluoro-[1,2-13C2]octanoic acid (13C2-PFOA)5.0020414.9370.0
10.Perfluorooctanoic acid (PFOA)5.0040413.1369.1
11.Perfluorononanoic acid (PFNA)5.6440463.1419.0
12.Perfluorooctanesulfonic acid (PFOS)5.6640499.280.1
PeakstR (min)Conc.
(ng/L)
Precursor IonProduct Ion
13.Perfluoro-1-[1,2,3,4-13C4]octanesulfonic acid (13C4-PFOS)5.6760503.180.2
14.9-Chlorohexadecafluoro-3-oxanone-1-sulfonic acid (9Cl-PF3ONS)5.9740531.0350.9
15.Perfluoro-n-[1,2-13C2]decanoic acid (13C2-PFDA)6.1820515.2470.1
16.Perfluorodecanoic acid (PFDA)6.1840512.9468.9
17.N-methyl perfluorooctanesulfonamidoacetic acid (N-MeFOSAA)6.3840570.2419.0
18.N-deuteriomethylperfluoro-1-octanesulfonamidoacetic acid (d3-N-MeFOSAA)6.3880573.1419.1
19.N-deuterioethylperfluoro-1-octanesulfonamidoacetic acid (d5-N-EtFOSAA)6.6180589.2419.1
20.N-ethyl perfluorooctanesulfonamidoacetic acid (N-EtFOSAA)6.6240583.8418.9
21.Perfluoroundecanoic acid (PFUnA)6.6440563.2519.1
22.11-Chloroeicosafluoro-3-oxaundecane-1-sulfonic acid (11Cl-PF3OUdS)6.8640630.8451.1
23.Perfluorododecanoic acid (PFDoA)7.0540613.1569.1
24.Perfluorotridecanoic acid (PFTrDA)7.4040663.0619.2
25.Perfluorotetradecanoic acid (PFTA)7.7140713.1669.0
ColumnRaptor C18 (cat.# 9304A52)
Dimensions:50 mm x 2.1 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Temp.:40 °C
Sample
Diluent:96:4 Methanol:water
Conc.:5-20 ng/mL in the final solution after sample preparation (equivalent to 20-80 ppt in laboratory reagent water sample prior to extraction)
Inj. Vol.:2 µL
Mobile Phase
A:Water, 5 mM ammonium acetate
B:Methanol
Time (min)Flow (mL/min)%A%B
0.000.47030
8.000.41090
8.010.47030
10.00.47030
DetectorMS/MS
Ion Mode:ESI-
Mode:MRM
InstrumentHPLC
NotesA PFAS delay column (cat.# 27854) was installed before the injector. The sample was prepared using Resprep S-DVB SPE cartridges (cat.# 28937) mounted on a Resprep vacuum manifold (cat.# 29298-VM) following the procedure in U.S. EPA Method 537.1. While internal standard concentrations varied, all target analytes were fortified at 40 ppt.