EPA Method 533 PFAS Calibration Standard

	Peaks	t_R (min)	Conc. (ng/mL)	Precursor Ion	Product Ion
1.	Perfluoro-n-butanoic acid (PFBA)	1.933	25	213	169
2.	Perfluoro-3-methoxypropanoic acid (PFMPA)	2.544	25	229	85
3.	Perfluoro-n-pentanoic acid (PFPeA)	3.369	25	263	219
4.	Perfluorobutanesulfonic acid (PFBS)	3.594	25	299	80
5.	Perfluoro-4-methoxybutanoic acid (PFMBA)	3.669	25	279	85
6.	Perfluoro(2-ethoxyethane)sulfonic acid (PFEESA)	3.919	25	315	135
7.	Perfluoro-3,6-dioxaheptanoic acid (NFDHA)	4.084	25	295	201
8.	1H,1H,2H,2H-Perfluorohexane sulfonic acid (4:2 FTS)	4.129	25	327	307
9.	Perfluorohexanoic acid (PFHxA)	4.189	25	313	269
10.	Perfluoro-1-pentanesulfonic acid (PFPeS)	4.278	25	349	80
11.	Perfluoro(2-methyl-3-oxahexanoic) acid (HFPO-DA)	4.365	25	285	169
12.	Perfluoroheptanoic acid (PFHpA)	4.715	25	363	319

	Peaks	t_R (min)	Conc. (ng/mL)	Precursor Ion	Product Ion
13.	Perfluoro-1-hexanesulfonic acid (PFHxS)	4.750	25	399	80
14.	4,8-dioxa-3H-perfluorononanoic acid (ADONA)	4.78	25	277	251
15.	1H,1H,2H,2H-Perfluorooctane sulfonic acid (6:2 FTS)	5.096	25	427	407
16.	Perfluoro-1-heptanesulfonic acid (PFHpS)	5.132	25	449	80
17.	Perfluorooctanoic acid (PFOA)	5.115	25	413	369
18.	Perfluorooctanesulfonic acid (PFOS)	5.441	25	499	80
19.	Perfluorononanoic acid (PFNA)	5.439	25	463	419
20.	9-Chlorohexadecafluoro-3-oxanonane-1-sulfonic acid (9Cl-PF3ONS)	5.588	25	531	351
21.	1H,1H,2H,2H-Perfluorodecane sulfonic acid (8:2 FTS)	5.712	25	527	507
22.	Perfluorodecanoic acid (PFDA)	5.712	25	513	469
23.	Perfluoroundecanoic acid (PFUnA)	5.954	25	563	519
24.	11-Chloroeicosafluoro-3-oxaundecane-1-sulfonic acid (11Cl-PF3OUdS)	6.049	25	631	451
25.	Perfluorododecanoic acid (PFDoA)	6.158	25	613	569

Column

Force C18 (cat.# 9634252)

Dimensions:

50 mm x 2.1 mm ID

Particle Size:

1.8 µm

Pore Size:

100 Å

Temp.:

40 °C

Standard/Sample

EPA 533 PFAS calibration standard (cat.# 30736)

Diluent:

80:20 Methanol:water

Conc.:

25 ng/mL

Inj. Vol.:

3 µL

Mobile Phase

Water, 5 mM ammonium acetate

Methanol

Time (min)	Flow (mL/min)	%A	%B
0.00	0.4	70	30
6.00	0.4	5	95
6.60	0.4	5	95
6.61	0.4	80	20
7.50	0.4	80	20

Detector	Shimadzu 8045
Ion Source:	Electrospray
Ion Mode:	ESI-
Mode:	MRM
Instrument	Shimadzu Nexera X2
Notes	Branched isomers for PFOA, PFOS, and PFHxS labeled as peak number "a" and "b". PFAS delay column used (cat.# 27854).

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