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Ethinyl Estradiol on Roc C18 - USP Method

LC_EX0518
PeakstR (min)Conc.
(µg/mL)
1.Ethylparaben3.25250
2.Ethinyl estradiol5.410200
ColumnRoc C18 (cat.# 9534565)
Dimensions:150 mm x 4.6 mm ID
Particle Size:5 µm
Pore Size:100 Å
Temp.:30 °C
Sample
Diluent:Water:acetonitrile (50:50)
Inj. Vol.:25 µL
Mobile Phase
A:Water:acetonitrile (50:50)
Time (min)Flow (mL/min)%A
0.001.0100
8.001.0100
DetectorUV @ 280, 4 nm
InstrumentHPLC