Ethinyl Estradiol on Roc C18 - USP Method

LC_EX0518

	Peaks	t_R (min)	Conc. (µg/mL)
1.	Ethylparaben	3.252	50
2.	Ethinyl estradiol	5.410	200

Column

Roc C18 (cat.# 9534565)

Dimensions:

150 mm x 4.6 mm ID

Particle Size:

5 µm

Pore Size:

100 Å

Temp.:

30 °C

Standard/Sample

Diluent:

Water:acetonitrile (50:50)

Inj. Vol.:

25 µL

Mobile Phase

A:

Water:acetonitrile (50:50)

Time (min)	Flow (mL/min)	%A
0.00	1.0	100
8.00	1.0	100

Detector	UV @ 280, 4 nm
Instrument	HPLC

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