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Fat-Soluble Vitamins on Raptor ARC-18 by LC-MS/MS

LC_FF0537
PeakstR (min)Precursor IonProduct Ion
1.Retinol1.46269.493.3
2.Vitamin D23.52397.5379.6
3.Vitamin D33.58385.5367.5
4.γ-Tocopherol3.78417.6151.4
5.α-Tocopherol4.12431.7165.4
6.Vitamin K15.23451.4187.3
ColumnRaptor ARC-18 (cat.# 9314A12)
Dimensions:100 mm x 2.1 mm ID
Particle Size:2.7 µm
Temp.:40 °C
Sample
Diluent:Methanol
Conc.:100 ng/mL
Inj. Vol.:5 µL
Mobile Phase
A:0.1% Formic acid + 5 mM ammonium formate in water
B:0.1% Formic acid + 5 mM ammonium formate in methanol
Time (min)Flow (mL/min)%A%B
0.000.51090
4.00.50100
5.00.50100
5.010.51090
7.00.51090
Max Pressure:190 bar
DetectorABSCIEX API 4000
Ion Source:TurboIonSpray®
Ion Mode:ESI+
Mode:MRM
InstrumentShimadzu UFLCXR