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Flavonols on Raptor C18 by LC-MS/MS

LC_FF0557
PeakstR (min)Precursor IonProduct Ion
1.Rutin1.04611.5303.3
2.Quercitrin1.19449.4303.3
3.Myricetin1.26319.3153.2
4.Fisetin1.27287.3137.3
5.Morin1.36303.3153.2
6.Quercetin1.48303.3153.2
7.Kaempferol1.68287.3153.2
8.Isorhamnetin1.72317.3153.2
9.Rhamnetin1.91317.3123.3
10.Galangin2.15271.3153.2
ColumnRaptor C18 (cat.# 9304552)
Dimensions:50 mm x 2.1 mm ID
Particle Size:5 µm
Pore Size:90 Å
Temp.:40 °C
Sample
Diluent:90:10 (water:acetonitrile)
Conc.:500 ng/mL
Inj. Vol.:5 µL
Mobile Phase
A:0.1% Formic acid in water
B:0.1% Formic acid in acetonitrile
Time (min)Flow (mL/min)%A%B
0.000.59010
2.400.53070
2.410.59010
3.500.59010
DetectorMS/MS
Ion Mode:ESI+
InstrumentHPLC