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Analysis of Isoflavones on Force C18 by UHPLC-MS

LC_FF0566
PeakstR (min)SIR (m/z)
1.Daidzin0.622417.2
2.Genistin0.949433.2
3.Daidzein1.174255.1
4.Genistein1.314271.1
ColumnForce C18 (cat.# 9634252)
Dimensions:50 mm x 2.1 mm ID
Particle Size:1.8 µm
Temp.:50 °C
SampleCustom mix
Diluent:Water
Conc.:500 ng/mL
Inj. Vol.:2 µL
Mobile Phase
A:Water + 0.1% formic acid
B:Acetonitrile + 0.1% formic acid
Time (min)Flow (mL/min)%A%B
0.000.68515
1.500.6595
1.510.68515
3.000.68515
Max Pressure:500 bar
DetectorMS
Interface:ESI+
InstrumentUHPLC