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Analysis of 21 Cannabinoids on Raptor ARC-18 by LC-MS

LC_FF0583
PeakstR (min)Conc.
(ng/mL)
SIM ESI
1.Cannabidivarin (CBDV)1.44500287.0+
2.Cannabidivarinic acid (CBDVA)1.56500331.0+
3.Cannabidiol (CBD)2.14500315.0+
4.Cannabigerol (CBG)2.15500317.0+
5.Cannabidiolic acid (CBDA)2.26500357.0-
6.Tetrahydrocannabivarin (THCV)2.44500287.0+
7.Cannabigerolic acid (CBGA)2.70500359.0-
8.Cannabichromevarin (CBCV)3.15500287.0+
9.Cannabinol (CBN)3.43500311.0+
10.Cannabidiphorol (CBDP)3.62500343.5+
PeakstR (min)Conc.
(ng/mL)
SIM ESI
11.Tetrahydrocannabivarinic acid (THCVA)3.97500331.0+
12.Δ9-Tetrahydrocannabinol (Δ9-THC)4.25500315.0+
13.Δ8-Tetrahydrocannabinol (Δ8-THC)4.55500315.0+
14.Cannabinolic acid (CBNA)4.91500353.0-
15.Cannabicyclol (CBL)4.94500315.0+
16.Cannabichromene (CBC)5.73500315.0+
17.Tetrahydrocannabinolic acid A (THCA-A)7.52500357.0-
18.Cannabicitran (CBT)7.55500315.0+
19.Tetrahydrocannabiphorol (THCP)8.28500343.5+
20.Cannabichromenic acid (CBCA)8.31500357.0-
21.Cannabicyclolic acid (CBLA)8.79500357.0-
ColumnRaptor ARC-18 (cat.# 9314A62)
Dimensions:150 mm x 2.1 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Guard Column:Raptor ARC-18 EXP 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9314A0252)
Temp.:30 °C
Standard/SampleCannabidivarinic acid (cat.# 34134)
Cannabidivarin (cat.# 34123)
Cannabidiolic acid (cat.# 34094)
Cannabigerolic acid (cat.# 34135)
Cannabigerol (cat.# 34091)
Cannabidiol (cat.# 34011)
Tetrahydrocannabivarin (cat.# 34100)
Cannabinol (cat.# 34010)
d9-Tetrahydrocannabinol (cat.# 34067)
d8-Tetrahydrocannabinol (cat.# 34090)
Cannabicyclol (cat.# 34130)
Cannabichromene (cat.# 34092)
d9-Tetrahydrocannabinolic acid A (cat.# 34111)
Diluent:Water:acetonitrile (20:80)
Conc.:500 ppb ng/mL
Inj. Vol.:2 µL
Mobile Phase
A:Water, 0.1% formic acid, 12 mM ammonium formate
B:Acetonitrile:methanol (50:50), 0.1% formic acid
Time (min)Flow (mL/min)%A%B
0.000.52080
90.52080
DetectorMS
Acquisition Type:SIM
Interface:ESI+
InstrumentUHPLC