Welcome to the New Restek.com! Take a tour of the new site and feel free to send us your feedback.

Your web browser will no longer be supported by Restek.com as of 30 June 2021.
To avoid any interruption in access or functionality, install a current-generation web browser now. Learn more.

CBG Hemp Flower Sample on Raptor ARC-18 2.7 μm by HPLC-UV

ColumnRaptor ARC-18 (cat.# 9314A65)
Dimensions:150 mm x 4.6 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Guard Column:Raptor ARC-18 EXP guard column cartridge 5 mm, 4.6 mm ID, 2.7 µm (cat.# 9314A0250)
Temp.:30 °C
Diluent:25:75 Water:acetonitrile
Inj. Vol.:5 µL
Mobile Phase
A:Water, 5 mM ammonium formate, 0.1% formic acid
B:Acetonitrile, 0.1% formic acid
Time (min)Flow (mL/min)%A%B
DetectorUV/VIS @ 228 nm
Notes500 mg of ground CBG hemp flower was weighed into a 50 mL centrifuge tube. 10 mL of methanol:water (80:20) was added prior to vortexing (5 seconds) and sonicating (5 minutes) over a total of three cycles. The sample was then centrifuged for 5 minutes at 4200 rpm. The supernatant was diluted 20-fold in water:acetonitrile (25:75), vortexed briefly, and filtered using a 0.2 µm Thomson SINGLE StEP standard filter vial (cat.#: 25893) prior to analysis.