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Penicillin, Cephalosporin, and Tetracycline Antibiotics on Raptor C18 by LC-MS/MS

LC_FS0500
PeakstR (min)Conc.
(ng/mL)
Precursor IonProduct IonProduct Ion
1.Desacetyl cephapirin0.68150382.03111.92124.21
2.Amoxicillin0.89100366.24349.10208.07
3.Cephapirin1.0550424.17292.08124.14
4.Desfuroyl ceftiofur cysteine disulfide1.06300549.16183.02126.00
5.Cefquinome1.16200529.19134.10125.12
6.Ampicillin1.1850350.19106.07160.06
7.Cefalexin1.19100348.10158.05174.05
8.Oxytetracycline1.2650461.27426.15443.32
9.Cefalonium1.29100459.16337.03123.10
10.Tetracycline1.3250445.28154.07427.32
PeakstR (min)Conc.
(ng/mL)
Precursor IonProduct IonProduct Ion
11.Cefacetrile1.37300362.07258.08178.01
12.Cefazolin1.50100455.10323.06295.09
13.Chlortetracycline1.5450479.27154.07371.06
14.Cefoperazone1.69100646.26143.07148.02
15.Ceftiofur1.8550524.14241.08125.24
16.Penicillin G2.18100335.18176.07160.07
17.Penicillin V2.33100351.10160.06114.07
18.Oxacillin2.44100402.15160.05114.06
19.Cloxacillin2.56100436.15277.06160.05
20.Nafcillin2.6325415.19199.09171.06
21.Dicloxacillin2.76100470.11160.05311.02
ColumnRaptor C18 (cat.# 9304A12)
Dimensions:100 mm x 2.1 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Guard Column:Raptor C18 EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9304A0252)
Temp.:40 °C
Sample
Diluent:Water
Conc.:25–300 ng/mL
Inj. Vol.:2 µL
Mobile Phase
A:0.1% Formic acid in water
B:0.1% Formic acid in acetonitrile
Time (min)Flow (mL/min)%A%B
0.000.49010
3.000.42080
3.010.49010
5.000.49010
DetectorMS/MS
Ion Mode:ESI+
Mode:MRM
InstrumentUHPLC