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Ionophore Antibiotics on Raptor Biphenyl by LC-MS/MS

LC_FS0503
PeakstR (min)Conc.
(ng/mL)
Precursor IonProduct IonProduct Ion
1.Lasalocid A1.92100613.42377.28595.40
2.Monensin2.12100693.50675.44461.30
3.Salinomycin2.19100773.57431.24531.39
4.Maduramicin2.30100939.65877.58473.31
5.Narasin2.58100787.59431.27531.35
ColumnRaptor Biphenyl (cat.# 9309A12)
Dimensions:100 mm x 2.1 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Guard Column:Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252)
Temp.:40 °C
Sample
Diluent:Water:methanol (10:90)
Conc.:100 ng/mL
Inj. Vol.:2 µL
Mobile Phase
A:0.5% Formic acid in water
B:0.5% Formic acid in acetonitrile
Time (min)Flow (mL/min)%A%B
0.000.53565
3.000.52575
3.010.53565
5.000.53565
DetectorMS/MS
Ion Mode:ESI+
Mode:MRM
InstrumentUHPLC