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Amphenicol and Tetracycline Antibiotics on Raptor C18 by LC-MS/MS

LC_FS0504
PeakstR (min)Conc.
(ng/mL)
Precursor IonProduct IonProduct Ion
1.Oxytetracycline1.2825461.27426.15443.32
2.Tetracycline1.3425445.28154.07427.32
3.Thiamphenicol*1.48200354.16290.04184.98
4.Chlortetracycline1.5625479.27154.07371.06
5.Florfenicol*1.86200356.10336.02184.98
6.Chloramphenicol*1.95200321.16151.99257.04
*Acquired in negative ion mode.
ColumnRaptor C18 (cat.# 9304A12)
Dimensions:100 mm x 2.1 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Guard Column:Raptor C18 EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9304A0252)
Temp.:40 °C
Sample
Diluent:Water
Conc.:25–200 ng/mL
Inj. Vol.:2 µL
Mobile Phase
A:0.1% Formic acid in water
B:0.1% Formic acid in acetonitrile
Time (min)Flow (mL/min)%A%B
0.000.49010
3.000.42080
3.010.49010
5.000.49010
DetectorMS/MS
Ion Mode:ESI+/ESI-
Mode:MRM
InstrumentUHPLC
NotesTetracyclines and amphenicols were analyzed with ESI+ and ESI- mode, respectively.