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Paraquat and Diquat on Raptor HILIC-Si

LC_FS0512
PeakstR (min)Precursor Ion 1Product Ion 1Precursor Ion 2Product Ion 2
1.Diquat3.25183.3157.2183.3130.1
2.Paraquat3.46185.3170.3171.377.2
ColumnRaptor HILIC-Si (cat.# 9310A52)
Dimensions:50 mm x 2.1 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Temp.:45 °C
SampleParaquat & diquat calibration mix (cat.# 32437)
Diluent:Mobile phase B
Conc.:50 ng/mL (cat.# 32437 diluted to 50 ng/mL in mobile phase B)
Inj. Vol.:5 µL
Mobile Phase
A:Water, 50 mM ammonium formate, 0.5% formic acid
B:25:75 Water:acetonitrile, 50 mM ammonium formate, 0.5% formic acid
Time (min)Flow (mL/min)%A%B
0.000.60100
4.000.63565
4.010.60100
7.000.60100
DetectorMS/MS
Ion Mode:ESI+
Mode:MRM
InstrumentHPLC