Welcome to the New Restek.com! Take a tour of the new site and feel free to send us your feedback.
Your web browser will no longer be supported by Restek.com as of 30 June 2021.
To avoid any interruption in access or functionality, install a current-generation web browser now. Learn more.

Mycotoxins in Peanut Powder on Raptor Biphenyl by LC-MS/MS

LC_FS0526
PeakstR (min)Conc.
(ng/g)
Precursor IonProduct Ion 1Product Ion 2
1.Deoxynivalenol0.6250297.3249.3231.2
2.Fumonisin B12.4550722.5352.4334.5
3.HT-22.6050447.3345.3285.3
4.Fumonisin B32.8550706.5336.4318.4
5.Fumonisin B23.2350706.5336.3141.2
6.T23.3150489.3245.2387.4
PeakstR (min)Conc.
(ng/g)
Precursor IonProduct Ion 1Product Ion 2
7.Aflatoxin G23.745331.2313.3189.3
8.Zearalenone3.9650319.3283.3187.2
9.Aflatoxin G14.225329.2243.2200.2
10.Aflatoxin B24.435315.3287.3259.2
11.Aflatoxin B14.995313.3285.2241.2
12.Ochratoxin A5.195404.2239.3358.3
Matrix interference was observed for HT-2 transition 447.3-285.3 in peanut powder. Therefore, 447.3-345.3 was chose for quantification.
ColumnRaptor Biphenyl (cat.# 9309A52)
Dimensions:50 mm x 2.1 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Guard Column:Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252)
Temp.:40 °C
Inj. Vol.:5 µL
Mobile Phase
A:Water, 2 mM ammonium formate, 0.1% formic acid
B:Methanol, 2 mM ammonium formate, 0.1% formic acid
Time (min)Flow (mL/min)%A%B%C
0.000.57030
0.60.57030
0.70.55050
3.00.52570
4.50.52575
5.00.51090
5.20.51090
5.210.52575
6.000.52575
6.010.57030
7.000.57030
DetectorMS/MS
Ion Mode:ESI+
Mode:MRM
InstrumentUHPLC
NotesWeighed 1.00 gram of peanut powder in a 50 mL centrifuge tube and added 2.00 mL of water. Vortexed at 3000 rpm for 5 min followed by the addition of 4.0 mL of extraction solvent (50:50 water:acetonitrile, v/v). The tube was then vortexed at 3000 rpm for 5 min followed by centrifugation for 15 min at 4200 rpm. 475 μL of the supernatant was filtered through a Thomson SINGLE StEP Nano filter vial (0.2 μm, cat.# 25882). The sample was then fortified with 25 μL of a standard solution prepared in water at 1000 ng/mL (100 ng/mL for aflatoxins and ochratoxin A) as part of the matrix-matched calibration curve. Vortexed at 3000 rpm for 1 min prior to analysis.