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Sulfur Antibiotics by UHPLC on Pinnacle DB Biphenyl

LC_GN0532
PeakstR (min)
1.Sulfanilamide1.55
2.Sulfadiazine3.74
3.Sulfapyridine4.09
4.Sulfathiazole4.24
5.Sulfamerazine4.35
PeakstR (min)
6.Sulfamethazine4.91
7.Sulfachlorpyridazine5.87
8.Sulfadoxine5.99
9.Sulfisoxazole6.37
10.Sulfadimethoxine7.14
11.Sulfaquinoxaline7.40
ColumnPinnacle DB Biphenyl (cat.# 9409212)
Dimensions:100 mm x 2.1 mm ID
Particle Size:1.9 µm
Pore Size:140 Å
Temp.:25 °C
Sample
Diluent:0.1% Formic acid in water
Conc.:50 µg/mL
Inj. Vol.:2 µL
Mobile Phase
A:0.1% Formic acid in water
B:0.1% Formic acid in acetonitrile
Time (min)%A%B
0.00955
86040
Flow:0.4 mL/min
DetectorUV/Vis @ 265 nm
InstrumentShimadzu UFLCXR