16 Cannabinoids on Raptor ARC-18 2.7 μm by LC-UV

	Peaks	t_R (min)
1.	Cannabidivarinic acid (CBDVA)	1.877
2.	Cannabidivarin (CBDV)	2.086
3.	Cannabidiolic acid (CBDA)	2.592
4.	Cannabigerolic acid (CBGA)	2.750
5.	Cannabigerol (CBG)	2.912
6.	Cannabidiol (CBD)	3.084
7.	Tetrahydrocannabivarin (THCV)	3.391
8.	Tetrahydrocannabivarinic acid (THCVA)	4.279

	Peaks	t_R (min)
9.	Cannabinol (CBN)	4.609
10.	Cannabinolic acid (CBNA)	5.437
11.	Δ9-Tetrahydrocannabinol (Δ9-THC)	5.815
12.	Δ8-Tetrahydrocannabinol (Δ8-THC)	6.002
13.	Cannabicyclol (CBL)	6.916
14.	Cannabichromene (CBC)	7.263
15.	Tetrahydrocannabinolic acid A (THCA-A)	7.612
16.	Cannabichromenic acid (CBCA)	8.510

Column

Raptor ARC-18 (cat.# 9314A65)

Dimensions:

150 mm x 4.6 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Guard Column:

Raptor ARC-18 EXP guard column cartridge, 2.7 µm (cat.# 9314A0250)

Temp.:

30 °C

Standard/Sample

Tetrahydrocannabivarin (cat.# 34100)

Cannabidiolic acid (CBDA) (cat.# 34094)

Cannabichromene (CBC) (cat.# 34092)

Cannabigerol (CBG) (cat.# 34091)

delta-9-Tetrahydrocannabinolic acid A (THCA-A) (cat.# 34093)

delta-8-Tetrahydrocannabinol (Δ⁸-THC) (cat.# 34090)

delta-9-Tetrahydrocannabinol (Δ⁹-THC) (cat.# 34067)

Cannabinol (CBN) (cat.# 34010)

Cannabidiol (CBD) (cat.# 34011)

Compounds not present in these mixes were obtained separately.

Diluent:

25:75 Water:methanol

Conc.:

50 µg/mL

Inj. Vol.:

5 µL

Mobile Phase

Water, 5 mM ammonium formate, 0.1% formic acid

Acetonitrile, 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	1.5	25	75
9.00	1.5	25	75

Detector	UV/Vis @ 228 nm
Instrument	HPLC

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