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Potency Analysis of a Commercially Available CBD Product on Raptor ARC-18 2.7 μm by LC-UV

ColumnRaptor ARC-18 (cat.# 9314A65)
Dimensions:150 mm x 4.6 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Guard Column:Raptor ARC-18 EXP guard column cartridge 2.7 µm (cat.# 9314A0250)
Temp.:30 °C
Sample
Diluent:25:75 Water:methanol
Conc.: 6.24 mg/mL (CBD)
Inj. Vol.:5 µL
Mobile Phase
A:Water, 5 mM ammonium formate, 0.1% formic acid
B:Acetonitrile, 0.1% formic acid
Time (min)Flow (mL/min)%A%B
0.001.52575
9.001.52575
DetectorUV/Vis @ 228 nm
InstrumentHPLC
Notes50 μL of a commerically available CBD product was diluted in 950 μL of 25:75 water:methanol. The sample was vortexed for 30 seconds at 3000 rpm. 400 μL of the sample was then filtered using a Thomson SINGLE StEP standard filter vial (PVDF, 0.2 μm, Restek cat.# 25895) prior to analysis. CBDA, CBG, CBD, Δ9-THC, and CBC were detected, but only CBD was quantified.