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|Column||Raptor ARC-18 (cat.# 9314A65)|
|Dimensions:||150 mm x 4.6 mm ID|
|Particle Size:||2.7 µm|
|Pore Size:||90 Å|
|Guard Column:||Raptor ARC-18 EXP guard column cartridge 2.7 µm (cat.# 9314A0250)|
|Conc.:||6.24 mg/mL (CBD)|
|Inj. Vol.:||5 µL|
|A:||Water, 5 mM ammonium formate, 0.1% formic acid|
|B:||Acetonitrile, 0.1% formic acid|
|Detector||UV/Vis @ 228 nm|
|Notes||50 μL of a commerically available CBD product was diluted in 950 μL of 25:75 water:methanol. The sample was vortexed for 30 seconds at 3000 rpm. 400 μL of the sample was then filtered using a Thomson SINGLE StEP standard filter vial (PVDF, 0.2 μm, Restek cat.# 25895) prior to analysis. CBDA, CBG, CBD, Δ9-THC, and CBC were detected, but only CBD was quantified.|