Cannabis Flower Sample on Raptor ARC-18 2.7 μm by HPLC-UV

	Peaks	t_R (min)
1.	Cannabidivarinic acid (CBDVA)	2.72
2.	Cannabigerolic acid (CBGA)	2.90
3.	Tetrahydrocannabivarinic acid (THCVA)	4.45
4.	Δ9-Tetrahydrocannabinol (Δ9-THC)	6.05
5.	Tetrahydrocannabinolic acid A (THCA-A)	7.87

Column

Raptor ARC-18 (cat.# 9314A65)

Dimensions:

150 mm x 4.6 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Guard Column:

Raptor ARC-18 EXP guard column cartridge, 2.7 µm (cat.# 9314A0250)

Temp.:

30 °C

Standard/Sample

Diluent:

25:75 Water:methanol

Inj. Vol.:

5 µL

Mobile Phase

Water, 5 mM ammonium formate, 0.1% formic acid

Acetonitrile, 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	1.5	25	75
9.00	1.5	25	75

Detector	UV/Vis @ 228 nm
Instrument	HPLC
Sample Preparation	200 mg of ground flower was weighed into a 50 mL centrifuge tube. 10 mL of IPA was added prior to vortexing (5 seconds) and sonicating (5 minutes) over a total of three cycles. The sample was then centrifuged for 5 minutes at 3000 rpm. The supernatant was diluted 10-fold in 25:75 water:methanol, vortexed briefly, and filtered using a 0.2 μm Thomson SINGLE StEP standard filter vial (cat.#: 25893) prior to analysis.

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