Welcome to the New Restek.com! Take a tour of the new site and feel free to send us your feedback.

Your web browser will no longer be supported by Restek.com as of 30 June 2021.
To avoid any interruption in access or functionality, install a current-generation web browser now. Learn more.

Cannabis Concentrate Sample on Raptor ARC-18 1.8 μm by UHPLC-UV

LC_GN0592
ColumnRaptor ARC-18 (cat.# 931421E)
Dimensions:100 mm x 3.0 mm ID
Particle Size:1.8 µm
Pore Size:90 Å
Guard Column:UltraShield UHPLC precolumn filter (cat.# 25809)
Temp.:30 °C
Sample
Diluent:25:75 Water:methanol
Inj. Vol.:2 µL
Mobile Phase
A:Water, 5 mM ammonium formate, 0.1% formic acid
B:Acetonitrile, 0.1% formic acid
Time (min)Flow (mL/min)%A%B
0.001.02575
4.001.02575
DetectorUV/Vis @ 228 nm
InstrumentUHPLC
Notes20 mg of concentrate was weighed into a 50 mL centrifuge tube. 10 mL of ethanol was added prior to vortexing (5 seconds) and sonicating (5 minutes) over a total of three cycles. The sample was then centrifuged for 5 minutes at 3000 rpm. The supernatant was diluted 50-fold in 25:75 water:methanol, vortexed briefly, and filtered using a 0.2 μm Thomson SINGLE StEP standard filter vial (cat.#: 25893) prior to analysis.