Welcome to the New Restek.com! Take a tour of the new site and feel free to send us your feedback.

Your web browser will no longer be supported by Restek.com as of 30 June 2021.
To avoid any interruption in access or functionality, install a current-generation web browser now. Learn more.

Blank Chocolate Spiked with Six Cannabinoids (0.2 mg/g) on Raptor ARC-18

LC_GN0673
PeakstR (min)Conc. (µg/mL)*
1.Cannabidiolic acid (CBDA)2.183.33
2.Cannabigerol (CBG)2.443.33
3.Cannabidiol (CBD)2.583.33
4.Cannabinol (CBN)3.833.33
5.Delta-9-Tetrahydrocannabinol (Δ9-THC)4.833.33
6.Tetrahydrocannabinolic acid A (THCA-A) 6.423.33
*Extract from a chocolate sample initially spiked at 0.2 mg/g.
ColumnRaptor ARC-18 (cat.# 9314A62)
Dimensions:150 mm x 2.1 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Guard Column:Raptor ARC-18 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9314A0252)
Temp.:30 °C
Standard/SampleCannabinoids standard (cat.# 34014)
Cannabigerol (cat.# 34091)
d9-Tetrahydrocannabinol (cat.# 34067)
d9-Tetrahydrocannabinolic acid A (cat.# 34111)
Diluent:75:25 Acetonitrile:water
Conc.: Expected concentration of 3.33 ppm in final extract from chocolate initially spiked at 0.2 mg/g.
Inj. Vol.:2 µL
Mobile Phase
A:Water, 5 mM ammonium formate, 0.1% formic acid
B:Acetonitrile, 0.1% formic acid
Time (min)Flow (mL/min)%A%B
0.000.42575
10.000.42575
DetectorUV/Vis @ 228 nm
InstrumentUHPLC
NotesChocolate was pulverized using a SPEX Freezer/Mill grinder, and a 0.5 g sample was fortified with cannabinoids at 0.2 mg/g. Then, 0.5 mL of isopropyl alcohol was added to the sample. The sample was vortexed for 10 sec or until a homogenous mixture was obtained. Afterwards, 2.5 mL of acetonitrile acidified with acetic acid at 1% v/v was added to the vial. Once again, the mixture was vortexed for 30 sec, and then centrifuged for 5 min at 4300 ×g at room temperature. 100 µL of chocolate extract was mixed with 900 µL of 75:25 acetonitrile:water, and the final mix was centrifuged for 10 min at 4 ⁰C. 2 µL of final extract was injected into the HPLC-UV system.