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Analysis of 13 Alkaloids Found in Psychedelic Mushrooms Using Force Biphenyl by HPLC-UV

ColumnForce Biphenyl (cat.# 962931E)
Dimensions:100 mm x 3.0 mm ID
Particle Size:3 µm
Pore Size:100 Å
Guard Column:Force Biphenyl EXP Guard Cartridge 5 mm, 3.0 mm ID (cat.# 962950253)
Temp.:60 °C
Conc.:50 ppm
Inj. Vol.:1 µL
Mobile Phase
A:Water, 10 mM ammonium formate, 0.1% formic acid
B:Methanol, 0.1% formic acid
Time (min)Flow (mL/min)%A%B
DetectorUV/Vis @ 222 nm
Flow Cell Size:500 nL
InstrumentWaters ACQUITY UPLC H-Class
Sample PreparationStandards were prepared in a 2.0 mL amber, short-cap vial (cat.# 21142) with a 250 µL glass insert (cat.# 24518) and capped with a 9 mm short cap (cat.# 24497).

NotesSystem and column were passivated using LC Passivation Solution (cat.# 32475). The outlined method conditions were used to perform five injections of passivation solution (5 µL) on column, diverting to waste. Five warm-up injections were completed prior to analysis.

Storage Conditions: Flush column using acetonitrile with 0.1% formic acid for 5 min.