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Vitamins B1, B2, B3, and B6 on Ultra C8

PeakstR (min)
1.Vitamin B3 (nicotinamide)2.732
2.Vitamin B6 (pyridoxine)5.615
3.Vitamin B2 (riboflavin)8.719
4.Vitamin B1 (thiamine)19.004
ColumnUltra C8 (cat.# 9103575)
Dimensions:250 mm x 4.6 mm ID
Particle Size:5 µm
Pore Size:100 Å
Temp.:50 °C
Diluent:Water:methanol (80:20)
Conc.:50 µg/mL each component
Inj. Vol.:5 µL
Mobile Phase0.008M sodium 1-hexanesulfonate:methanol:glacial acetic acid:triethylamine (817.5:175:7.5:0.2)
Flow:2.0 mL/min
DetectorUV/Vis @ 270 nm
InstrumentShimadzu UFLCXR