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Featured Application: Carbamate Pesticides on an Ultra Carbamate LC Column

Analyze Carbamate Pesticides in a Fraction of the Time and Boost Productivity

  • Ultra Carbamate LC columns equip you to analyze more samples per shift.
  • Separate target carbamates in just 5 minutes with a total cycle time of 8 minutes by LC-MS/MS or LC-UV.
  • Pair with convenient and reliable Restek certified reference materials for accurate, precise results.
 

Carbamate pesticides are widely used insecticides that pose human and environmental health risks due to their activity as endocrine disruptors. While many pesticides can be analyzed successfully using GC, carbamate pesticides are thermally unstable and degrade quickly in the hot GC inlet. Therefore, LC methods must be used to monitor levels of these semivolatile compounds in soil and water samples. C18 columns can be used for carbamate pesticides analysis, but traditional methods—such as EPA Method 531—require analysis times of over 20 minutes.

Using an Ultra Carbamate column instead of a C18 is a much better approach as this column was developed specifically for carbamates and allows much faster analysis times. The column has a unique selectivity and is compatible with UV, fluorescence, or LC-MS detection. As shown below, commonly monitored carbamate pesticides can be separated in less than 5 minutes (8 minutes total cycle time) using LC-MS/MS and a 100 mm x 2.1 mm Ultra Carbamate column. Alternately, LC-UV detection at 220 nm may be used. Using an Ultra Carbamate column provides much faster analysis times than are typically achieved using a C18 column, which allows more samples to be analyzed per shift. In addition to increased sample throughput, this analysis will significantly reduce solvent usage—and the costs of disposing of solvent waste.

LC_EV0534
PeakstR (min)Precursor IonProduct IonProduct Ion
1.Aldicarb sulfone1.77240.186.2148.2
2.Aldicarb sulfoxide1.92207.1132.189.1
3.Oxamyl2.01237.172.190.1
4.Methomyl2.22163.1106.088.1
5.3-Hydroxycarbofuran2.68238.1163.0181.0
6.Aldicarb3.44208.1116.089.0
7.Propoxur3.71210.1111.0168.1
8.Carbofuran3.80222.1165.1123.0
9.Carbaryl4.07202.1145.0127.0
10.Methiocarb4.78226.1169.1121.1
ColumnUltra Carbamate (cat.# 9177312)
Dimensions:100 mm x 2.1 mm ID
Particle Size:3 µm
Pore Size:100 Å
Temp.:30 °C
Sample531.1 Carbamate pesticide calibration mixture (cat.# 32273)
Diluent:Water
Conc.:10 ng/mL
Inj. Vol.:5 µL
Mobile Phase
A:0.1% Formic acid + 5 mM ammonium formate in water
B:0.1% Formic acid + 5 mM ammonium formate in methanol
Time (min)Flow (mL/min)%A%B
0.000.57525
5.000.5595
5.010.57525
8.000.57525
DetectorMS/MS
Ion Mode:ESI+
Mode:MRM
InstrumentHPLC
EVSS2394A-UNV

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