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Effects of Column Inner Diameter and Packed Bed Heterogeneities on Chromatographic Performance

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Authors: Edward G. Franklin and Ty W. Kahler
Restek Corporation

Published By: LCGC North America

Year of Publication: 2016


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In recent years, industry has been moving to columns with smaller and smaller inner diameters—moving from 4.6 and 3.0 mm i.d. columns to 2.1 mm, 1.0 mm, and even smaller. While small inner diameter columns have some clear advantages, they also bring challenges.  Reduction of extracolumn volumes must be given greater consideration by both customers and manufacturers. This installment of “Column Watch” focuses on the sources of band broadening within high performance liquid chromatography (HPLC) columns with an emphasis on eddy dispersion. The physical mechanisms of dispersion are discussed and a review of the current literature as it pertains to small inner diameter columns is presented.