We get it. Your market is quickly changing and you need a chromatography partner that understands that. Whether you are part of a well-established safety and potency lab or starting a new lab, Restek has the products and expertise you need for successful cannabis analyses. Being an employee-owned and independent chromatography company, every employee at Restek has a vested interest in your success. We design the best solutions for your lab, regardless of the instrumentation and techniques used. Here, you will find innovative LC and GC products and methodologies designed to fit your toughest analytical problems.
We’ve been in your shoes. That’s why we understand your challenges and focus on solving them. Using our expertise to develop innovative products that help chromatographers has always been, and continues to be, Restek’s top priority. We strive to develop industry-leading technologies that fit the needs of today’s analysts. When setting up a laboratory for cannabis testing, we realize that you need dependable products that deliver high-quality data without considerable capital investment. We know you need to work with a company that understands the challenges of your market and supports you with tailored solutions and superior customer service.
PRODUCT POTENCY TESTING
Our High-Throughput LC and GC Cannabinoids Methods Produce Results Quickly without the Cost of New Equipment
When setting up a lab, often you just can’t invest in the latest instrumentation, but you still need to get results fast. We understand that. That’s why Restek has developed both LC and GC methods for cannabinoids that let you report potency results quickly. For LC, we created a fast analysis of 16 cannabinoids that can be performed on any LC instrument. By utilizing Raptor column technology, as shown in Figure 1, we developed a nine-minute analysis that is compatible with any HPLC instrument—so you get UHPLC speed on your existing equipment without the capital investment. Also, we specifically chose an easy-to-make mobile phase that can be directly transferred to LC-MS, if you ever need to move to MS due to regulation changes. For labs using GC equipment, you can analyze cannabinoids in just minutes using an Rxi-35Sil MS column and the instrument conditions shown in Figure 2. Why did we focus on fast cannabinoid analyses? Potency testing is the cornerstone of your lab. Building a fast method means your productivity increases and you can analyze more samples per day on the same instrument, delaying the need for expensive capital investments in new equipment.
Figure 1: Raptor LC columns give you fast analysis times for cannabinoids without the expense of UHPLC equipment.
Using Thomson SINGLE StEP filter vials is an ideal way to remove particulate matter that could clog your column. They provide sample filtration that’s economical, eco-friendly, and fast!
Reduce Capital Investments—Analyze Terpenes by GC on the Same Setup Used for Residual Solvents
Cannabis has a complex terpene profile, which is theorized to increase its therapeutic effects. Terpene profiling is used for both product quality testing and strain identification. These complex and sometimes problematic compounds are challenging to analyze, but the experts at Restek have developed qualitative GC methodology for terpene profiling that fits easily into required laboratory workflows. To keep things simple, the GC terpene profile analysis in Figure 3 can be performed on the same instrument and column that we recommend for residual solvent testing.
Figure 3: Comprehensive terpene analysis by headspace GC-FID can be done on the same instrument and GC column as residual solvents analysis, which simplifies setup and improves lab productivity.
Did you know that headspace analysis eliminates the possibility of column contamination from nonvolatile matrix components? This results in an extremely clean chromatogram, minimal instrument maintenance, and longer column lifetimes.
RESIDUAL SOLVENT ANALYSIS
Improve Productivity—Keep Analyzing Samples Instead of Changing Columns between Residual Solvent and Terpene Methods.
As the popularity of cannabis grows, so does concern over the safety of the drug products. Cannabis concentrates can contain residual solvents left over from manufacturing that can be harmful to human health. Because of this risk, many states will require residual solvent testing of cannabis concentrates. Due to their high volatility, residual solvents can only be analyzed using GC techniques. The chemists at Restek have developed a quick and easy method that allows for residual solvent analysis (Figure 4) and terpene profiling (Figure 3) on the same column and instrument platform with minimal sample preparation.
Figure 4: Improve productivity and reduce downtime for column changes—this sensitive headspace GC-FID analysis of residual solvents can be accomplished on the same instrument and Rxi-624Sil MS column that is used in Restek’s terpenes profiling method.
You’ll save money ordering from Restek because we understand the need to control costs and build efficient workflows. We develop as many analyses as possible using the same columns and consumables, so you can minimize the number of products you need to stock.
Ensure Product Safety with Fast, Selective Multiresidue Pesticide Analysis
In addition to residual solvents, cannabis products can contain residues of pesticides that were applied to cannabis plants during growth in order to control agricultural pests. These pesticides can be analyzed by LC-MS/MS, GC-MS/MS, and GC-MS. Regardless of the technique used, lists of target compounds can be extensive, so column selectivity is an important factor in achieving good separations. Both Raptor ARC-18 LC columns (Figure 5) and Rxi-5ms GC columns (Figure 6) provide the selectivity needed for accurate and reliable multiresidue pesticides analysis. Removing matrix interferences while also recovering the analytes of interest is also crucial for a successful pesticide analysis using either LC or GC, and Restek’s Q-sep QuEChERS products allow for fast, easy, adaptable cleanup of a wide variety of matrices.
Figure 5: A high-throughput separation of 204 pesticides by LC-MS/MS can be achieved in only 7 minutes with the Raptor ARC-18 column.
Methanol + 2 mM ammonium formate + 0.2% formic acid
Waters Xevo TQ-S
Waters Zspray™ ESI
Waters ACQUITY UPLC I-Class
When combining a large number of compounds with different chemical functionalities, mix stability can be an issue. In formulating our LC multiresidue pesticide standard kit (cat.# 31971), we extensively studied the 204 compounds involved, then grouped them into as few mixes as possible while still ensuring maximum long-term stability and reliability. Several of these compounds are isomeric and separation of the isomers accounts for 216 peaks in the chromatogram compound list. For quantitative analysis, we recommend analyzing each mix separately to ensure accurate results for every compound.
Figure 6: Rxi-5ms GC columns reliably separate many commonly used pesticides.
By Breaking Boundaries in Our Industry, We Help You Succeed in Yours
Restek has been helping cannabis labs establish innovative, cost-effective analytical solutions from the very beginning, and we will continue to help you manage your ever changing analytical challenges every step of the way.
High Throughput Analysis of Cannabinoids in CBD Oil by LC UV