Regulated Carcinogenic Aryl Amines Resulting From Reductive Cleavage of Azo Dyes on Rxi-35Sil MS

	Peaks	t_R (min)	Conc. (µg/mL)	Molecular Ion (m/z)
1.	o-Toluidine	3.15	10	107
2.	o-Anisidine	4.36	10	123
3.	4-Chloroaniline	4.77	10	127
4.	p-Cresidine	5.46	10	137
5.	2,4,5-Trimethylaniline	5.52	10	135
6.	3-Chloro-o-toluidine	5.84	10	141
7.	4-Chloro-o-toluidine	5.96	10	141
8.	2,4-Diaminotoluene	7.58	10	122
9.	2,4,5-Trichloroaniline (IS)	9.13	30	196
10.	2-Naphthylamine	9.72	10	143
11.	2-Aminobiphenyl	9.84	10	169
12.	2-Amino-4-nitrotoluene	10.49	10	152

	Peaks	t_R (min)	Conc. (µg/mL)	Molecular Ion (m/z)
13.	4-Aminobiphenyl	12.12	10	169
14.	p-Aminoazobenzene	15.93	10	197
15.	4,4'-Oxydianiline	16.60	10	200
16.	4,4'-Diaminodiphenylmethane	16.70	10	198
17.	Benzidine	16.77	10	184
18.	o-Aminoazotoluene	17.52	10	225
19.	3,3'-Dimethyl-4,4'-diaminodiphenylmethane	18.23	10	226
20.	3,3'-Dimethylbenzidine	18.48	10	212
21.	4,4’-Thiodianiline	19.43	10	216
22.	3,3'-Dichlorobenzidine	19.79	10	252
23.	4,4'-Methylenebis(2-chloroaniline)	19.79	10	266
24.	3,3'-Dimethoxybenzidine	19.91	10	244

Elution order of positional isomers was verified with individual standards.

Injection
Column	Rxi-35Sil MS, 30 m, 0.25 mm ID, 0.25 µm (cat.# 13823)
Standard/Sample	AccuStandard carcinogenic aryl amine mix (cat.# AE-000-49-R1)

Diluent:	Ethyl acetate
Conc.:	10 µg/mL
Inj. Vol.:	1 µL split (split ratio 10:1)
Liner:	Premium 4 mm Precision liner w/wool (cat.# 23305.1)
Inj. Temp.:	275 °C
Oven
Oven Temp.:	100 °C to 320 °C at 9.5 °C/min (hold 5 min)
Carrier Gas	He, constant flow
Flow Rate:	2.0 mL/min

Detector

Mode:

Scan

Scan Program:

Group	Start Time (min)	Scan Range (amu)	Scan Rate (scans/sec)
1	2.0	30-300	5.5

Transfer Line Temp.:

330 °C

Analyzer Type:

Quadrupole

Source Type:

Inert

Drawout Plate:

3 mm ID

Source Temp.:

250 °C

Quad Temp.:

180 °C

Electron Energy:

70 eV

Solvent Delay Time:

2.0 min

Tune Type:

PFTBA

Ionization Mode:

Instrument

Agilent 7890A GC & 5975C MSD

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