Psilocybin and Psilocin on Raptor HILIC-Si by LC-MS/MS

	Peaks	t_R (min)	Conc. (ng/mL)	Precursor Ion	Product Ion 1	Product Ion 2
1.	Psilocin	1.12	100	205.1	160.1	115.0
2.	Psilocybin	1.73	100	285.1	240.0	205.2

Column

Raptor HILIC-Si (cat.# 9310A52)

Dimensions:

50 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Temp.:

40 °C

Standard/Sample

Diluent:

10 mM Ammonium formate in 90:10 acetonitrile:water (v/v)

Conc.:

100 ng/mL

Inj. Vol.:

2 µL

Mobile Phase

Water, 10 mM ammonium formate

90:10 Acetonitrile:water (v/v), 10 mM ammonium formate

Time (min)	Flow (mL/min)	%A	%B
0.00	0.5	0	100
0.2	0.5	0	100
1.70	0.5	95	5
1.71	0.5	0	100
3.00	0.5	0	100

Detector	MS/MS
Ion Source:	Electrospray
Ion Mode:	ESI+
Mode:	MRM
Sample Preparation	A 100 ng/mL standard mix of psilocin and psilocybin was prepared in 10 mM ammonium formate in 90:10 acetonitrile:water (v/v). The solution was vortexed at 3000 rpm for 10 seconds to mix, and then the supernatant was injected for LC-MS/MS analysis.

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