Sulfur Antibiotics by UHPLC on Pinnacle DB Biphenyl

LC_GN0532

	Peaks	t_R (min)
1.	Sulfanilamide	1.55
2.	Sulfadiazine	3.74
3.	Sulfapyridine	4.09
4.	Sulfathiazole	4.24
5.	Sulfamerazine	4.35

	Peaks	t_R (min)
6.	Sulfamethazine	4.91
7.	Sulfachlorpyridazine	5.87
8.	Sulfadoxine	5.99
9.	Sulfisoxazole	6.37
10.	Sulfadimethoxine	7.14
11.	Sulfaquinoxaline	7.40

Column

Pinnacle DB Biphenyl (cat.# 9409212)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

1.9 µm

Pore Size:

140 Å

Temp.:

25 °C

Standard/Sample

Diluent:

0.1% Formic acid in water

Conc.:

50 µg/mL

Inj. Vol.:

2 µL

Mobile Phase

A:

0.1% Formic acid in water

B:

0.1% Formic acid in acetonitrile

Time (min)	%A	%B
0.00	95	5
8	60	40

Flow:

0.4 mL/min

Detector	UV/Vis @ 265 nm
Instrument	Shimadzu UFLCXR

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