PFAS Analysis by U.S. EPA Method 1633 for Aqueous, Solid, Biosolids, and Tissue
- Sample Matrix: aqueous, solid, biosolids, and tissue samples
- Sample Preparation: WAX solid-phase extraction
- Detection Technique: LC-MS/MS
Analysis of Per- and Polyfluoroalkyl Substances (PFAS) in Aqueous, Solid, Biosolids, and Tissue Samples by LC-MS/MS
U.S. EPA Method 1633 is used for the analysis of 40 PFAS in aqueous, solid, biosolids, and tissue samples. The analysis of bile acids TCDCA, TDCA, and TUDCA is also required. EPA Method 1633 combines solid-phase extraction sample preparation using weak anion exchange (WAX) cartridges with LC-MS/MS analysis on a C18 column. The method is performance based and allows modifications to be made to improve performance (e.g., to overcome interferences or improve sensitivity, accuracy, or precision), but only if all method performance criteria are met. The full analyte list and recommended products for PFAS analysis in aqueous, solid, biosolids, and tissue samples by Method 1633 are provided below. In addition, you can register and directly compare analyte lists and matrices across PFAS analysis methods here or visit www.restek.com/PFAS to explore our technical resources for PFAS analysis.
Analyte List for U.S. EPA Method 1633 for PFAS Analysis
PFAS acronyms, compound names, and CAS numbers vary across methods and guidelines, and they also depend on compound form (e.g., neutral vs. salt). The following list uses standardized terminology to allow easy comparison across the methods and guidelines in our comprehensive guide. Please refer to U.S. EPA Method 1633 for method-specific nomenclature. Note that the analysis of bile acids TCDCA, TDCA, and TUDCA is also required by this method.
Acronym | Compound Name | CAS# |
NEtFOSAA | N-ethyl perfluorooctanesulfonamidoacetic acid | 2991-50-6 |
NMeFOSAA | N-methyl perfluorooctanesulfonamidoacetic acid | 2355-31-9 |
PFBS | Perfluoro-1-butanesulfonic acid | 375-73-5 |
PFDA | Perfluorodecanoic acid | 335-76-2 |
PFDoA | Perfluorododecanoic acid | 307-55-1 |
PFHpA | Perfluoroheptanoic acid | 375-85-9 |
PFHxS | Perfluoro-1-hexanesulfonic acid | 355-46-4 |
PFHxA | Perfluorohexanoic acid | 307-24-4 |
PFNA | Perfluorononanoic acid | 375-95-1 |
PFOS | Perfluorooctanesulfonic acid | 1763-23-1 |
PFOA | Perfluorooctanoic acid | 335-67-1 |
PFTeDA | Perfluorotetradecanoic acid | 376-06-7 |
PFTrDA | Perfluorotridecanoic acid | 72629-94-8 |
PFUnA | Perfluoroundecanoic acid | 2058-94-8 |
HFPO-DA, GenX | Hexafluoropropylene oxide dimer acid | 13252-13-6 |
PFBA | Perfluorobutanoic acid | 375-22-4 |
PFPeA | Perfluoropentanoic acid | 2706-90-3 |
PFDS | Perfluoro-1-decanesulfonic acid | 335-77-3 |
PFHpS | Perfluoro-1-heptanesulfonic acid | 375-92-8 |
PFPeS | Perfluoro-1-pentanesulfonic acid | 2706-91-4 |
FOSA | Perfluoro-1-octanesulfonamide | 754-91-6 |
4:2 FTS | 1H,1H,2H,2H-Perfluorohexane sulfonic acid | 757124-72-4 |
6:2 FTS | 1H,1H,2H,2H-Perfluorooctane sulfonic acid | 27619-97-2 |
8:2 FTS | 1H,1H,2H,2H-Perfluorodecane sulfonic acid | 39108-34-4 |
NMeFOSA | N-Methylperfluorooctanesulfonamide | 31506-32-8 |
NEtFOSA | N-ethylperfluorooctanesulfonamide | 4151-50-2 |
9Cl-PF3ONS | 9-Chlorohexadecafluoro-3-oxanonane-1-sulfonic acid (F-53B Major) | 756426-58-1 |
11Cl-PF3OUdS | 11-Chloroeicosafluoro-3-oxaundecane-1-sulfonic acid (F-53B Minor) | 763051-92-9 |
ADONA | 4,8-Dioxa-3H-perfluorononanoic acid | 919005-14-4 |
PFNS | Perfluoro-1-nonanesulfonic acid | 68259-12-1 |
PFEESA | Perfluoro(2-ethoxyethane)sulfonic acid | 113507-82-7 |
NFDHA | Nonafluoro-3,6-dioxaheptanoic acid | 151772-58-6 |
PFMPA | Perfluoro-3-methoxypropanoic acid | 377-73-1 |
PFMBA | Perfluoro-4-methoxybutanoic acid | 863090-89-5 |
NMeFOSE | 2-(N-methylperfluoro-1-octanesulfonamido)-ethanol | 24448-09-7 |
NEtFOSE | 2-(N-ethylperfluoro-1-octanesulfonamido)-ethanol | 1691-99-2 |
PFDoS | Perfluorododecane sulfonate | 79780-39-5 |
3:3 FTCA | 3:3 Fluorotelomer carboxylic acid | 356-02-5 |
5:3 FTCA | 5:3 Fluorotelomer carboxylic acid | 914637-49-3 |
7:3 FTCA, FHpPA | 7:3 Fluorotelomer carboxylic acid or 3-perfluoroheptyl propanoic acid | 812-70-4 |
Recommended Products for U.S. EPA Method 1633 for PFAS Analysis
ANALYSIS
Analytical LC Columns:
- Raptor C18 SPP column: 2.7 μm, 50 x 2.1 mm (cat.# 9304A52)
- Force C18 FPP column: 1.8 μm, 50 x 2.1 mm (cat.# 9634252)
Delay Columns:
- PFAS delay column: 5 µm, 50 x 2.1 mm (cat.# 27854)
- PFAS delay column: 5 µm, 50 x 3.0 mm (cat.# 580959 [custom product])
Guard Columns:
- UltraShield UHPLC precolumn filter: 0.2 µm frit (cat.# 25809)
- EXP direct connect holder for EXP guard cartridges (cat.# 25808)
- Raptor C18 EXP guard column cartridge: 2.7 µm, 5 x 2.1 mm (cat.# 9304A0252)
SAMPLE HANDLING
Sample Prep:
- 30 mm syringe filter: 0.45 µm, nylon (cat.# 23981)
- 30 mm syringe filter: 0.22 µm, nylon (cat.# 23980)
- Deactivated borosilicate glass wool (cat.# 20789)
- Thomson SINGLE StEP standard filter vials with screw-top caps: 0.2 µm, nylon w/preslit cap (cat.# 27896)
- Resprep PFAS SPE cartridge: 6 mL, WAX 150 mg/GCB 50 mg (cat.# 28930)
- Resprep PFAS SPE cartridge with filter aid: 6 mL, filter aid 2000 mg/WAX 150 mg/GCB 50 mg (cat.# 28931)
- Resprep polymeric SPE cartridge: WAX, 6 mL/500 mg, 30 µm (cat.# 28268)
- Resprep polymeric SPE cartridge: WAX, 6 mL/200 mg, 30 µm (cat.# 28292)
- Q-sep multispeed centrifuge for QuEChERS (cat.# 28295)
Sample Reservoirs:
Vacuum Manifolds:
Vacuum Pumps:
- Chemker 300 PTFE vacuum pump: 20 L/min, AC110 V, 60 Hz (cat.# 27426)
- Chemker 300 PTFE vacuum pump: 18 L/min, AC220 V, 50 Hz (cat.# 27427)
GENERAL LAB SUPPLIES
- Polypropylene vials: 100-pk. (cat.# 23243)
- Polypropylene vials: 1000-pk. (cat.# 23246)
- Polyethylene vial caps: 100-pk. (cat.# 23244)
- Polyethylene vial caps: 1000-pk. (cat.# 23247)
- Centrifuge tubes: 50 mL, 50-pk. (cat.# 25846)
- Centrifuge tubes: 50 mL, 500-pk. (cat.# 28290)
- Norm-Ject plastic syringe: 10 mL Luer Lock tip (cat.# 22775)
- Norm-Ject plastic syringe: 5 mL Luer Lock tip (cat.# 22774)
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