Viva
Viva Biphenyl, 5 µm, 250 x 4.6 mm HPLC Column
Viva Biphenyl, 5 µm, 250 x 4.6 mm HPLC Column
Overview
- Excellent for separating peptides or proteins.
- Rugged, spherical particles with 300 Å pore size.
- High proportion of pore/surface area available to large molecules.
Guard column requires Trident LC column protection system or Trident HPLC in-line guard cartridge holder.
Viva columns are based on a wide pore material we designed for optimal large-molecule separations. In developing Viva silica, we found that although many commercial wide-pore silicas meet the standard 300 Å mean pore size, most have very broad distributions about this mean, with a significant portion of their pore volume falling below 150 Å. This means a large portion of the surface area is unavailable to larger molecules. Viva columns have a narrow distribution around the mean pore size, permitting a larger portion of the silica surface to play a role in the separation.
Since 2005, the Restek Biphenyl has offered a greater degree of dispersion than conventional phenyls and a greater degree of polarizability than phenyl hexyls, creating higher selectivity and a greater range of usability. Because of these heightened interactions, this column shows substantial increases in retention—especially for dipolar, unsaturated, or conjugated solutes—and enhanced orthogonal selectivity when using methanol mobile phases. It is ideal for increasing sensitivity and selectivity in LC-MS analyses.
Stationary Phase Category: phenyl (L11)
Ligand Type: Biphenyl
Particle: 5 µm, spherical
Pore Size: 300 Å
Carbon Load: 7%
End-Cap: yes
Surface Area: 100 m2/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C