Accurately Analyze Metal-Sensitive Compounds with Restek’s New Inert LC Columns

Our new inert LC column technology helps labs improve their analysis of metal-sensitive compounds. A premium inert coating applied to the stainless-steel surface of our LC columns reduces nonspecific binding of chelating analytes, enabling sensitive analysis and smooth integration of peaks. Combined with Restek’s selective stationary phases, these new inert LC columns are ideal for the analysis of metal-sensitive compounds, such as organophosphorus pesticides and mycotoxins.

Restek’s inert LC columns can provide four key benefits:

• Improved peak shape without passivation or mobile phase additives.
• Increased response and analyte recovery, allowing lower detection limits.
• High accuracy and throughput with less variability.
• Less time-consuming conditioning and complicated passivation required.

Exceptional Inertness Brings Exceptional Performance

Analyzing compounds that have nonspecific adsorption (NSA) or nonspecific binding (NSB) to metal surfaces in LC columns have historically been a challenge. Poor peak shape and sensitivity are key indicators that polar, usually acidic compounds are interacting with the metal surfaces in the column, causing poor data quality. Our premium inert column technology is designed to eliminate NSA and NSB of active analytes to the column hardware, giving analysts greater confidence in their data when working with metal-sensitive analytes.

Pesticides

Pesticide panels benefit from the use of inert LC columns as they contain a wide variety of compounds. Phosphorylated, acidic, polar compounds, and/or metal chelating species, such as organophosphate pesticides, are reactive to the metal surfaces inside of the analytical column. Our new inert LC columns solve that problem easily to improve the overall performance of your pesticide panel.

Figure 1: Our new Raptor inert ARC-18 columns offer increased sensitivity, recoveries, and lower detection limits without any column preconditioning.

	Peaks	tR (min)	Precursor Ion	Product Ion 1	Product Ion 2	Peak Area	Peak Height
1.	Methamidophos	1.33	142.0	94.0	125.1	428941	105189
2.	Acephate	1.55	184.0	143.0	48.9	300642	104729
3.	Omethoate	1.72	214.0	125.0	182.9	892008	337690
4.	Monocrotophos	2.21	224.1	127.0	193.1	215810	78425
5.	Dicrotophos	2.35	238.1	112.1	72.0	404916	159292
6.	Dimethoate	2.52	230.0	125.0	199.0	807805	342939
7.	Trichlorfon	2.53	257.0	108.9	220.8	173942	63266
8.	Vamidothion	2.54	288.0	146.0	118.0	1333829	547308
9.	Mevinphos isomer 1	2.55	241.9	126.9	192.9	311274	129961
10.	Mevinphos isomer 2	2.76	241.9	126.9	192.9	74030	29802
11.	Carbaryl	3.18	202.1	145.0	127.0	39671	11924
12.	Isocarbophos	3.52	291.1	231.1	121.1	33294	11941
13.	Dimethomorph isomer 1	3.96	388.2	300.9	165.1	511766	172977
14.	Dimethomorph isomer 2	4.13	388.2	300.9	165.1	877031	328826
15.	Temephos	5.70	467.1	124.9	418.9	164310	64751

Column	Raptor Inert ARC-18 (cat.# 9314A12-T)
Dimensions:	100 mm x 2.1 mm ID
Particle Size:	2.7 µm
Pore Size:	90 Å
Temp.:	50 °C
Standard/Sample	LC multiresidue pesticide standard #1 (cat.# 31972)
Diluent:	Water, 0.1% formic acid
Conc.:	1 ng/mL
Inj. Vol.:	5 µL
Mobile Phase
A:	Water, 2 mM ammonium formate, 0.1% formic acid
B:	Methanol, 2 mM ammonium formate, 0.1% formic acid
	Time (min) Flow (mL/min) %A %B 0.00 0.4 95 5 2.00 0.4 40 60 4.00 0.4 25 75 6.00 0.4 0 100 7.50 0.4 0 100 7.51 0.4 95 5 9.00 0.4 95 5

Detector	Shimadzu LCMS-8060
Ion Mode:	ESI+
Mode:	MRM
Instrument	Shimadzu Nexera X2

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Figure 2: Compared to a conventional, stainless-steel column, our new Raptor inert ARC-18 columns provide exceptional analyte recoveries.

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Figure 3: Peak area and peak height are greatly improved for the analysis of pesticides.

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Table I: Restek's new inert columns showed up to 2X improvement in peak area and peak height over stainless-steel columns in this analysis of pesticides.

Mycotoxins

Mycotoxins analysis can be challenging and often requires a great deal of column conditioning and equilibration to achieve acceptable peaks. This is due to the reactive nature of the compounds, which contain acidic, polar, or otherwise metal chelating groups. Our new inert column hardware, combined with our stationary phases, helps simplify methods and improve the response and peak shape of these compounds.

Figure 4: Achieve excellent peak shape without additional acid passivation or mobile phase additives when analyzing mycotoxins with our new Raptor inert biphenyl columns.

	Peaks	tR (min)	Conc. (ng/mL)	Precursor Ion	Product Ion	Peak Area	Peak Height
1.	Nivalenol	0.88	10	295.1	137.1	4182	64495
2.	Deoxynivalenol	1.25	10	297.2	231.0	17346	281906
3.	Fusarenon-X	1.92	10	355.1	137.1	7668	121790
4.	15-Acetyldeoxynivalenol	3.08	10	339.2	137.1	31369	517570
5.	3-Acetyldeoxynivalenol	3.14	10	339.2	213.1	22613	296396
6.	Tenuazonic acid	4.11	10	198.1	125.0	47828	197658
7.	Altenuene	4.60	10	293.2	257.1	113850	2059699
8.	Alternariol	5.27	10	259.0	185.1	73272	1302192
9.	Ergosine	5.28	10	548.4	208.1	486620	9366601
10.	Citrinin	5.46	10	251.2	233.1	1007880	9828889
11.	Ergosinine	5.46	10	548.4	208.1	496734	8740527
12.	Fumonisin B1	5.48	10	722.5	352.3	122878	2415567
13.	Diacetoxyscirpenol	5.62	10	384.2	247.1	68139	1208825
14.	Ergotamine	5.71	10	582.4	223.2	493003	9274155
15.	Ergocornine	5.85	10	562.4	268.2	387025	7732744
16.	Ergotaminine	5.96	10	582.4	223.2	462119	9237991
17.	HT-2	6.13	10	447.2	345.1	15221	323765
18.	Ergocryptine	6.19	10	576.4	268.2	522204	11360838
19.	Fumonisin B3	6.23	10	706.4	336.2	143302	3444421
20.	Ergocristine	6.44	10	610.4	223.2	195562	4450058
21.	Fumonisin B2	6.59	10	706.4	336.2	151719	3869822
22.	Tentoxin	6.62	10	415.2	312.2	95175	2131906
23.	α-Zearalenol	6.91	10	303.1	285.1	30224	702420
24.	Ergocorninine	6.93	10	562.4	268.2	704029	14389283
25.	Aflatoxin G2	6.97	10	331.2	189.0	262824	5274353
26.	T-2	7.09	10	489.2	387.1	56535	1394735
27.	Ergocryptinine	7.18	10	576.4	268.2	778972	16765348
28.	Ergocristinine	7.40	10	610.4	223.2	1583053	32975663
29.	Aflatoxin G1	7.45	10	329.1	199.7	304389	6102959
30.	Zearalenone	7.59	10	319.2	283.1	37162	927455
31.	Alternariol monomethylether	7.62	10	273.0	199.1	31024	640689
32.	Aflatoxin B2	7.63	10	315.1	287.0	295648	5724754
33.	Aflatoxin B1	8.02	10	313.2	241.1	223520	4425821
34.	Ochratoxin A	8.25	10	404.1	239.0	190060	4411953

Column	Raptor Inert Biphenyl (cat.# 9309A12-T)
Dimensions:	100 mm x 2.1 mm ID
Particle Size:	2.7 µm
Pore Size:	90 Å
Temp.:	60 °C
Standard/Sample	Aflatoxins standard (cat.# 34121)
	Ochratoxin A standard (cat.# 34122)
Diluent:	50:50 Water:methanol
Conc.:	10 ng/mL
Inj. Vol.:	5 µL
Mobile Phase
A:	Water, 0.05% formic acid
B:	Methanol, 0.05% formic acid
	Time (min) Flow (mL/min) %A %B 0.00 0.4 75 25 5.00 0.4 50 50 9.00 0.4 0 100 9.01 0.4 75 25 11.0 0.4 75 25
Max Pressure:	440 bar

Detector	Waters Xevo TQ-S
Ion Mode:	ESI+
Mode:	MRM
Instrument	Waters ACQUITY UPLC I-Class
Notes

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Figure 5: In this analysis of fumonisins, our new Raptor inert biphenyl columns achieve a dramatic increase in peak area compared to conventional, stainless-steel columns.

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Figure 6: Peak area and peak height are greatly improved for the analysis of fumonisins.

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Table II: In this analysis of mycotoxins, our new inert columns can provide an up to 10X increase in peak height over conventional columns.

Exceptional Inertness Meets Exceptional Stationary Phases

We’re introducing our inert LC column technology on three column types: Raptor Biphenyl, Raptor ARC-18, and Force Biphenyl. These new columns help bring the benefits of inert column technology to labs specializing in small molecule LC-MS/MS workflows.

Raptor LC Columns

Raptor LC columns combine the speed of 2.7 µm SPP with the resolution of Ultra Selective Liquid Chromatography (USLC) technology, improving separations and speeding up analysis times with standard HPLC instruments. When speed is your goal, Restek recommends the Raptor line of LC columns.

Learn more at www.restek.com/Raptor

Raptor Inert Biphenyl

The industry-leading Biphenyl is our most popular LC stationary phase. It is particularly adept at separating compounds that are hard to resolve or that elute early on C18 and other phenyl chemistries.

Raptor Inert ARC-18

The Raptor ARC-18 column features a well-balanced retention profile without the drawbacks of using an ordinary C18 in the harsh, acidic mobile phases needed for mass spectrometry. Even after extended use in these low-pH (≤ 2.0) conditions, the sterically protected ARC-18 offers consistent retention, peak shape, and response for charged bases, neutral acids, small polar compounds, and more.

Force LC Columns

Force fully porous particle (FPP) LC columns are designed and manufactured to handle high-pressure, high-stress conditions. They’re long-lasting, reproducible, and premium quality—backed by our 100% Pure Satisfaction guarantee. When you need greater retention and sharper peaks, Force LC columns are ready to be put to work.

Learn more at www.restek.com/Force

Force Inert Biphenyl

The Force Inert Biphenyl column separates compounds that other phenyl and C18 chemistries can’t. They allow the use of simple, MS-friendly mobile phases and are ideal for when you need to increase retention of hydrophilic aromatics.

Exceptional Inertness for Your Analysis

As chromatographers, we understand the importance of having confidence in your data. Whether you’re analyzing pesticides, mycotoxins, or any analysis containing metal-sensitive compounds, these new LC columns provide the accuracy, throughput, and performance your lab needs.

Contact your local Restek representative today at www.restek.com/contact-us to pair the benefits of our inert technology with your analysis.