Food Industry FAMEs on Rt-2560 by AOAC Method 996.06 Using H2

GC_FF1346

	Peaks	t_R (min)	Conc. (µg/mL)	Structural Nomenclature
1.	Methyl butyrate	6.621	400	C4:0
2.	Methyl caproate	7.711	400	C6:0
3.	Methyl octanoate	9.556	400	C8:0
4.	Methyl decanoate	12.094	400	C10:0
5.	Methyl undecanoate	13.506	200	C11:0
6.	Methyl dodecanoate	14.943	400	C12:0
7.	Methyl tridecanoate	16.372	200	C13:0
8.	Methyl myristate	17.772	400	C14:0
9.	Methyl myristoleate	18.916	200	C14:1 (c9)
10.	Methyl pentadecanoate	19.127	200	C15:0
11.	Methyl pentadecenoate	20.252	200	C15:1 (c10)
12.	Methyl palmitate	20.436	600	C16:0
13.	Methyl palmitoleate	21.361	200	C16:1 (c9)
14.	Methyl heptadecanoate	21.691	200	C17:0
15.	Methyl heptadecenoate	22.595	200	C17:1 (c10)
16.	Methyl stearate	22.899	400	C18:0
17.	Methyl octadecenoate	23.442	200	C18:1 (t9)
18.	Methyl oleate	23.677	400	C18:1 (c9)

	Peaks	t_R (min)	Conc. (µg/mL)	Structural Nomenclature
19.	Methyl linolelaidate	24.292	200	C18:2 (t9,t12)
20.	Methyl linoleate	24.811	200	C18:2 (c9,c12)
21.	Methyl arachidate	25.171	400	C20:0
22.	Methyl linolenate	25.637	200	C18:3 (c6,c9,c12)
23.	Methyl eicosenoate	25.903	200	C20:1 (c11)
24.	Methyl linolenate	26.081	200	C18:3 (c9,c12,c15)
25.	Methyl heneicosanoate	26.239	200	C21:0
26.	Methyl eicosadienoate	26.980	200	C20:2 (c11,c14)
27.	Methyl behenate	27.274	400	C22:0
28.	Methyl eicosatrienoate	27.763	200	C20:3 (c8,c11,c14)
29.	Methyl erucate	27.981	200	C22:1 (c13)
30.	Methyl eicosatrienoate	28.190	200	C20:3 (c11,c14,c17)
31.	Methyl arachidonate	28.286	200	C20:4 (c5,c8,c11,c14)
32.	Methyl tricosanoate	28.381	200	C23:0
33.	Methyl docosadienoate	29.071	200	C22:2 (c13,c16)
34.	Methyl lignocerate	29.341	400	C24:0
35.	Methyl eicosapentaenoate	29.737	200	C20:5 (c5,c8,c11,c14,c17)
36.	Methyl nervonate	30.131	200	C24:1 (C15)
37.	Methyl docosahexaenoate	33.132	200	C22:6 (c4,c7,c10,c13,c16,c19)

Injection
Column	Rt-2560, 100 m, 0.25 mm ID, 0.20 µm (cat.# 13198)
Standard/Sample	Food industry FAME mix (cat.# 35077)

Diluent:	Hexane
Conc.:	10,000 µg/mL total concentration
Inj. Vol.:	1 µL split (split ratio 20:1)
Liner:	Topaz 4.0 mm ID Precision inlet liner w/wool (cat.# 23305)
Inj. Temp.:	250 °C
Oven
Oven Temp.:	100 °C (hold 1.05 min) to 240 °C at 5.3 °C/min (hold 8.5 min)
Carrier Gas	H₂, constant flow
Flow Rate:	1.75 mL/min

Detector	FID @ 250 °C
Constant Column + Constant Make-up:	52 mL/min
Hydrogen flow:	40 mL/min
Air flow:	400 mL/min
Data Rate:	50 Hz
Instrument	Agilent 7890A GC
Notes	Resolution of all peaks >1.5 Method translated from He using the EZGC method translator (www.restek.com/FAMEH₂).

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